Document Detail


Regulation of rat-kidney cortex fructose-1,6-bisphosphatase activity. I. Effects of fructose-2,6-bisphosphate and divalent cations.
MedLine Citation:
PMID:  8138035     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The native rat-kidney cortex Fructose-1,6-BPase is differentially regulated by Mg2+ and Mn2+. 2. Mg2+ binding to the enzyme is hyperbolic and large concentrations of the cation are non-inhibitory. 3. Mn2+ produces a 10-fold rise in Vmax higher than Mg2+. [Mn2+]0.5 is much larger than [Mg2+]0.5. At elevated [Mn2+] inhibition is observed. 4. Mg2+ and Mn2+ produce antagonistic effects on the inhibition of the enzyme by high substrate. 5. Fru-2,6-P2 inhibits the enzyme by rising the S0.5 and favouring a sigmoidal kinetics. 6. The inhibition by Fru-2,6-P2 is released by Mg2+ and more powerfully by Mn2+ increasing the I0.5.
Authors:
M M Sola; F J Oliver; R Salto; M Gutiérrez; A M Vargas
Related Documents :
318415 - Hexokinase of angiostrongylus cantonensis: presence of a glucokinase.
24948565 - Design and optimization of highly-selective fungal cyp51 inhibitors.
6136275 - The role of specific cations in regulation of cyanobacterial glutamine synthetase.
1673335 - Activation of intestinal brush border guanylate cyclase by aromatic disulphide compounds.
3327525 - Catalytic mechanism and inhibition of trna (uracil-5-)methyltransferase: evidence for c...
19448135 - Cyp2d6-cyp2c9 protein-protein interactions and isoform-selective effects on substrate b...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  25     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-04-22     Completed Date:  1994-04-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1963-8     Citation Subset:  IM    
Affiliation:
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Granada, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Fructose-Bisphosphatase / antagonists & inhibitors,  drug effects*
Fructosediphosphates / pharmacology*
Kidney Cortex / drug effects*,  enzymology
Kinetics
Magnesium / pharmacology*
Male
Manganese / pharmacology*
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Fructosediphosphates; 7439-95-4/Magnesium; 7439-96-5/Manganese; 79082-92-1/fructose 2,6-diphosphate; EC 3.1.3.11/Fructose-Bisphosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Characterization of the type of carbohydrate chains of the higher molecular weight (140 kDa) acid ph...
Next Document:  Regulation of rat-kidney cortex fructose-1,6-bisphosphatase activity. II. Effects of adenine nucleot...