Document Detail

Regulation of rat-kidney cortex fructose-1,6-bisphosphatase activity. I. Effects of fructose-2,6-bisphosphate and divalent cations.
MedLine Citation:
PMID:  8138035     Owner:  NLM     Status:  MEDLINE    
1. The native rat-kidney cortex Fructose-1,6-BPase is differentially regulated by Mg2+ and Mn2+. 2. Mg2+ binding to the enzyme is hyperbolic and large concentrations of the cation are non-inhibitory. 3. Mn2+ produces a 10-fold rise in Vmax higher than Mg2+. [Mn2+]0.5 is much larger than [Mg2+]0.5. At elevated [Mn2+] inhibition is observed. 4. Mg2+ and Mn2+ produce antagonistic effects on the inhibition of the enzyme by high substrate. 5. Fru-2,6-P2 inhibits the enzyme by rising the S0.5 and favouring a sigmoidal kinetics. 6. The inhibition by Fru-2,6-P2 is released by Mg2+ and more powerfully by Mn2+ increasing the I0.5.
M M Sola; F J Oliver; R Salto; M Gutiérrez; A M Vargas
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  25     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-04-22     Completed Date:  1994-04-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1963-8     Citation Subset:  IM    
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Granada, Spain.
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MeSH Terms
Fructose-Bisphosphatase / antagonists & inhibitors,  drug effects*
Fructosediphosphates / pharmacology*
Kidney Cortex / drug effects*,  enzymology
Magnesium / pharmacology*
Manganese / pharmacology*
Rats, Wistar
Reg. No./Substance:
0/Fructosediphosphates; 7439-95-4/Magnesium; 7439-96-5/Manganese; 79082-92-1/fructose 2,6-diphosphate; EC

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