| Regulation of rRNA transcription correlates with nucleoside triphosphate sensing. | |
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MedLine Citation:
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PMID: 11591676 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously shown that the activity of the Escherichia coli rRNA promoter rrnB P1 in vitro depends on the concentration of the initiating nucleotide, ATP, and can respond to changes in ATP pools in vivo. We have proposed that this nucleoside triphosphate (NTP) sensing might contribute to regulation of rRNA transcription. To test this model, we have measured the ATP requirements for transcription from 11 different rrnB P1 core promoter mutants in vitro and compared them with the regulatory responses of the same promoters in vivo. The seven rrnB P1 variants that required much lower ATP concentrations than the wild-type promoter for efficient transcription in vitro were defective for response to growth rate changes in vivo (growth rate-dependent regulation). In contrast, the four variants requiring high ATP concentrations in vitro (like the wild-type promoter) were regulated with the growth rate in vivo. We also observed a correlation between NTP sensing in vitro and the response of the promoters in vivo to deletion of the fis gene (an example of homeostatic control), although this relationship was not as tight as for growth rate-dependent regulation. We conclude that the kinetic features responsible for the high ATP concentration dependence of the rrnB P1 promoter in vitro are responsible, at least in part, for the promoter's regulation in vivo, consistent with the model in which rrnB P1 promoter activity can be regulated by changes in NTP pools in vivo (or by hypothetical factors that work at the same kinetic steps that make the promoter sensitive to NTPs). |
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Authors:
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M M Barker; R L Gourse |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 183 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-10-09 Completed Date: 2001-12-04 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 6315-23 Citation Subset: IM |
Affiliation:
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Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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pharmacology Base Sequence Carrier Proteins / genetics Escherichia coli / genetics*, growth & development* Escherichia coli Proteins* Factor For Inversion Stimulation Protein Feedback Gene Expression Regulation, Bacterial* Guanosine Triphosphate / pharmacology Integration Host Factors Kinetics Mutation Promoter Regions, Genetic Purine Nucleotides / pharmacology* RNA, Bacterial / biosynthesis RNA, Ribosomal / biosynthesis* Sequence Alignment Transcription, Genetic rRNA Operon* |
| Grant Support | |
ID/Acronym/Agency:
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GM37048/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Escherichia coli Proteins; 0/Factor For Inversion Stimulation Protein; 0/Integration Host Factors; 0/Purine Nucleotides; 0/RNA, Bacterial; 0/RNA, Ribosomal; 0/integration host factor, E coli; 56-65-5/Adenosine Triphosphate; 86-01-1/Guanosine Triphosphate |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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