Document Detail

Regulation of rRNA synthesis and processing in animal cells. Effect of nucleoside analogues.
MedLine Citation:
PMID:  6212050     Owner:  NLM     Status:  MEDLINE    
The nucleoside analogues fluorouridine and fluorodeoxyuridine (both at 100 muM) and 8-azaguanine (at 500 muM) inhibit both rRNA transcription and processing in Ehrlich ascites cells. In BHK21 cells fluorodeoxyuridine has no effect on either rRNA maturation or transcription, whereas toyocamycin (at 2 microM) inhibits both processes in BHK21 cells and Ehrlich ascites cells. The drugs inhibit transcription in cells incubated in the complete medium, but have no effect on the decreased transcription in cells incubated in a medium without amino acids. This lack of effect cannot be explained by an altered uptake of the drugs in the amino acid-starved cells, since maturation of the rRNA precursor is affected in cells incubated in media with or without amino acids. The effect of the drugs on rRNA transcription is not the consequence of the inhibition of protein synthesis. The results lend support to the proposal that rRNA processing and transcription are co-ordinately controlled in cells with a high rate of rRNA synthesis.
S Iapalucci-Espinoza; M T Franze-Fernandez
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  202     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1982 Feb 
Date Detail:
Created Date:  1982-08-26     Completed Date:  1982-08-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  325-32     Citation Subset:  IM    
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MeSH Terms
Azaguanine / pharmacology*
Carcinoma, Ehrlich Tumor / metabolism
Cells, Cultured
Floxuridine / pharmacology*
Kidney / metabolism
RNA, Ribosomal / biosynthesis,  metabolism*
Ribosomal Proteins / biosynthesis
Toyocamycin / pharmacology
Transcription, Genetic / drug effects
Uracil Nucleotides / pharmacology*
Uridine / analogs & derivatives*,  pharmacology
Uridine Triphosphate / analogs & derivatives,  pharmacology*
Reg. No./Substance:
0/RNA, Ribosomal; 0/Ribosomal Proteins; 0/Uracil Nucleotides; 134-58-7/Azaguanine; 316-46-1/5-fluorouridine; 3828-96-4/5-fluorouridine 5'-triphosphate; 50-91-9/Floxuridine; 58-96-8/Uridine; 606-58-6/Toyocamycin; 63-39-8/Uridine Triphosphate

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