Document Detail

Regulation of proteoglycan and hyaluronan synthesis by elevated level of intracellular cyclic adenosine monophosphate in peritubular cells from immature rat testis.
MedLine Citation:
PMID:  9788747     Owner:  NLM     Status:  MEDLINE    
The effects of an increase in intracellular cAMP concentration on proteoglycan (PG) synthesis by peritubular (PT) cells from immature rat testis were investigated. In the presence of dBcAMP for 72 h, the [3H]-hexosamine incorporation in secreted PG and in cell-associated PG was reduced, whereas [35S]-sulfate radioactivity was enhanced in secreted PG and not affected in cell-associated PG. Cholera toxin and IBMX, known to generate high intracellular cAMP levels, induced similar changes. Cyclic AMP did not alter PG protein moiety synthesis but enhanced PG turnover. Cholera toxin and dBcAMP profoundly modified PG characteristics: (1) Apparent molecular weight of PG was increased. (2) This was due to an increase in glycosaminoglycans (heparan sulfate (HS) and chondroitin sulfate (CS)) length. (3) The number of glycosaminoglycan chains was presumably reduced. (4) Heparan sulfate and chondroitin sulfate chains of medium and cell layer-associated PG appeared oversulfated. (5) The pattern of cell layer associated PG was modified with a decrease in HSPG and a correlative increase in CSPG. Cholera toxin and dBcAMP also dramatically stimulated hyaluronan synthesis by possible phosphorylation induced activation of hyaluronan synthase(s).
B Thiébot; M Langris; P J Bonnamy; J Bocquet
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  187     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-12-18     Completed Date:  1998-12-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  99-112     Citation Subset:  IM    
Laboratoire de Biochimie, EP CNRS 009, IRBA, Université de Caen, France.
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MeSH Terms
1-Methyl-3-isobutylxanthine / pharmacology
Bucladesine / pharmacology
Cells, Cultured
Cholera Toxin / pharmacology
Chromatography, Gel
Cyclic AMP / metabolism*
Cyclic AMP-Dependent Protein Kinases / metabolism
DNA / analysis
Glycosaminoglycans / biosynthesis
Glycosides / pharmacology
Hyaluronic Acid / biosynthesis*
Phosphodiesterase Inhibitors / pharmacology
Proteoglycans / biosynthesis*
Rats, Sprague-Dawley
Testis / cytology,  metabolism*
Reg. No./Substance:
0/Glycosaminoglycans; 0/Glycosides; 0/Phosphodiesterase Inhibitors; 0/Proteoglycans; 2001-96-9/4-nitrophenyl beta-D-xyloside; 28822-58-4/1-Methyl-3-isobutylxanthine; 362-74-3/Bucladesine; 60-92-4/Cyclic AMP; 9004-61-9/Hyaluronic Acid; 9007-49-2/DNA; 9012-63-9/Cholera Toxin; EC AMP-Dependent Protein Kinases

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