| Regulation of proteoglycan and hyaluronan synthesis by elevated level of intracellular cyclic adenosine monophosphate in peritubular cells from immature rat testis. | |
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MedLine Citation:
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PMID: 9788747 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of an increase in intracellular cAMP concentration on proteoglycan (PG) synthesis by peritubular (PT) cells from immature rat testis were investigated. In the presence of dBcAMP for 72 h, the [3H]-hexosamine incorporation in secreted PG and in cell-associated PG was reduced, whereas [35S]-sulfate radioactivity was enhanced in secreted PG and not affected in cell-associated PG. Cholera toxin and IBMX, known to generate high intracellular cAMP levels, induced similar changes. Cyclic AMP did not alter PG protein moiety synthesis but enhanced PG turnover. Cholera toxin and dBcAMP profoundly modified PG characteristics: (1) Apparent molecular weight of PG was increased. (2) This was due to an increase in glycosaminoglycans (heparan sulfate (HS) and chondroitin sulfate (CS)) length. (3) The number of glycosaminoglycan chains was presumably reduced. (4) Heparan sulfate and chondroitin sulfate chains of medium and cell layer-associated PG appeared oversulfated. (5) The pattern of cell layer associated PG was modified with a decrease in HSPG and a correlative increase in CSPG. Cholera toxin and dBcAMP also dramatically stimulated hyaluronan synthesis by possible phosphorylation induced activation of hyaluronan synthase(s). |
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Authors:
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B Thiébot; M Langris; P J Bonnamy; J Bocquet |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 187 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 1998 Oct |
Date Detail:
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Created Date: 1998-12-18 Completed Date: 1998-12-18 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 99-112 Citation Subset: IM |
Affiliation:
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Laboratoire de Biochimie, EP CNRS 009, IRBA, Université de Caen, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Methyl-3-isobutylxanthine
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pharmacology Animals Bucladesine / pharmacology Cells, Cultured Cholera Toxin / pharmacology Chromatography, Gel Cyclic AMP / metabolism* Cyclic AMP-Dependent Protein Kinases / metabolism DNA / analysis Glycosaminoglycans / biosynthesis Glycosides / pharmacology Half-Life Hyaluronic Acid / biosynthesis* Male Phosphodiesterase Inhibitors / pharmacology Proteoglycans / biosynthesis* Rats Rats, Sprague-Dawley Testis / cytology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Glycosaminoglycans; 0/Glycosides; 0/Phosphodiesterase Inhibitors; 0/Proteoglycans; 2001-96-9/4-nitrophenyl beta-D-xyloside; 28822-58-4/1-Methyl-3-isobutylxanthine; 362-74-3/Bucladesine; 60-92-4/Cyclic AMP; 9004-61-9/Hyaluronic Acid; 9007-49-2/DNA; 9012-63-9/Cholera Toxin; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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