| Regulation of prolyl endopeptidase activity by the intracellular redox state. | |
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MedLine Citation:
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PMID: 2254307 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The activity of prolyl endopeptidase was markedly decreased during incubation of intact murine erythroleukemia cells at 45 degrees C, but not during incubation of sonicated cells or during incubation at 42 degrees C. The thermal inactivation of prolyl endopeptidase in situ required neither the synthesis of proteins and polynucleotides nor the synergistic activation of inhibitors. Moreover, inhibition of lysosomal proteinases and calpains or depletion of ATP did not affect the thermal inactivation of prolyl endopeptidase. This specific inactivation of prolyl endopeptidase was also observed following the addition to the culture medium of menadione or diamide, compounds known to increase intracellular oxidized glutathione levels. The activity of prolyl endopeptidase in the cell lysate was also dose-dependently decreased by the addition of glutathione disulfide and the decrease of the activity was prevented by coexistence of reduced glutathione. Furthermore, the level of intracellular oxidized glutathione was increased during incubation at 45 degrees C for 15 min, but not at 42 degrees C for 30 min. These results strongly suggest that the activity of prolyl endopeptidase is regulated by changes in the intracellular redox potential. |
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Authors:
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T Tsukahara; S Ishiura; H Sugita |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 265 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1990 Dec |
Date Detail:
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Created Date: 1991-01-23 Completed Date: 1991-01-23 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 21448-53 Citation Subset: IM |
Affiliation:
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National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Disulfides Endopeptidases / metabolism* Glutathione / metabolism* Hot Temperature Leukemia, Erythroblastic, Acute Mice Oxidation-Reduction* Proline Serine Endopeptidases* Stress, Physiological / enzymology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Disulfides; 147-85-3/Proline; 70-18-8/Glutathione; EC 3.4.-/Endopeptidases; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.26/prolyl oligopeptidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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