| Regulation of proliferation and differentiation of prostatic stromal cells by oestradiol through prostatic epithelial cells in a paracrine manner. | |
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MedLine Citation:
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PMID: 18190643 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To characterize a paracrine effect of prostatic epithelial cells in the presence or absence of oestradiol on the differentiation and proliferation of prostatic stromal cells. MATERIALS AND METHODS: Conditioned media (CM) collected from a prostatic epithelial cell line (BPH-1), which was pre-treated with different concentration of oestradiol, were added to cultures of primary prostatic stromal cells. The proliferation rates of stromal cells were determined using a tetrazolium assay. The mRNA level was analysed by real-time reverse transcription-polymerase chain reaction (RT-PCR), and the protein level of smooth muscle myosin heavy chain (SM-MHC), fibronectin and collagen IV were determined with Western blotting, enzyme- linked immunosorbent assay and radioimmunoassay, respectively. The expression of transforming growth factor beta1 (TGF beta 1) in the BPH-1 cell line was analysed. RESULTS: The rate of proliferation of stromal cells increased when they were cultured with CM harvested from oestradiol-treated BPH-1 cells, but there was no remarkable change when they were cultured with CM from untreated cells. The level of smoothelin mRNA and SM-MHC protein increased after treatment with CM from BPH-1. The CM from BPH-1 with oestradiol stimulation was more effective in stimulating smoothelin mRNA and SM-MHC protein level. The protein level of collagen type IV, but not fibronectin, was up-regulated in the supernatants and cell extracts of CM-treated stromal cells. Oestradiol enhanced the expression and secretion of TGF beta 1 in BPH-1 cells. TGF beta 1-neutralizing antibody abrogated the effect of BPH-1 CM on the synthesis of collagen IV and SM-MHC in stromal cells. CONCLUSION: These results suggest that oestradiol-stimulated proliferation and differentiation of prostatic stromal cells could be regulated by factors secreted from prostatic epithelial cells. |
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Authors:
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Quan Wu; Jiandang Shi; Linfeng Chen; Chun-Yu Wang; Irwin Park; Chung Lee; Ju Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-08 |
Journal Detail:
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Title: BJU international Volume: 101 ISSN: 1464-410X ISO Abbreviation: BJU Int. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-01-31 Completed Date: 2008-03-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100886721 Medline TA: BJU Int Country: England |
Other Details:
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Languages: eng Pagination: 497-502 Citation Subset: IM |
Affiliation:
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Bioactive Materials Key Laboratory of Ministry of Education, Institute for Molecular Biology, Nankai University, Tianjin, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western Case-Control Studies Cell Differentiation / drug effects* Cell Proliferation / drug effects Cells, Cultured Culture Media, Conditioned Enzyme-Linked Immunosorbent Assay Epithelial Cells / drug effects, pathology Estradiol / pharmacology* Extracellular Matrix / drug effects Humans Male Prostate / pathology* Prostatectomy Prostatic Hyperplasia / pathology* Reverse Transcriptase Polymerase Chain Reaction Stromal Cells / drug effects, pathology Transforming Growth Factor beta1 / metabolism* Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/Transforming Growth Factor beta1; 50-28-2/Estradiol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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