| Regulation of proliferation of LLC-MK2 cells by nucleosides and nucleotides: the role of ecto-enzymes. | |
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MedLine Citation:
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PMID: 8687400 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Using the incorporation of [methyl-3H]thymidine as a proliferation marker, the effects of various nucleosides and nucleotides on endothelial LLC-MK2 cells were studied. We found that ATP, ADP, AMP and adenosine in concentrations of 10 microM or higher stimulate the proliferation of these cells. 2. Inhibition of ecto-ATPase (EC 3.6.1.15), 5'-nucleotidase (EC 3.1.3.5) or alkaline phosphatase (EC 3.1.3.1) significantly diminished the stimulatory effect of ATP, indicating that the effect is primarily caused by adenosine and not by adenine nucleotides. Also, the effect depends only on extracellular nucleosides, since inhibition of nucleoside uptake by dipyridamole has no influence on proliferation. 3. Other purine nucleotides and nucleosides (ITP, GTP, inosine and guanosine) also stimulate cell proliferation, while pyrimidine nucleotides and nucleosides (CTP, UTP, cytidine and uridine) inhibit proliferation. Furthermore, the simultaneous presence of adenosine and any of the other purine nucleosides is not entirely additive in its effect on cell proliferation. At the same time any pyrimidine nucleoside, when added together with adenosine, has the same inhibitory effect as the pyrimidine nucleoside alone. 4. Apparently these proliferative effects are neither caused by any pharmacologically known P1-purinoceptor, nor are they mediated by cyclic AMP, cyclic GMP, or D-myo-inositol 1,4,5-trisphosphate as second messenger, nor by extracellular Ca2+. 5. Therefore, we conclude that various purine and pyrimidine nucleosides can influence the proliferation of LLC-MK2 cells by acting on putative purinergic and pyrimidinergic receptors not previously described. |
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Authors:
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R Lemmens; L Vanduffel; H Teuchy; O Culic |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 316 ( Pt 2) ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1996-08-20 Completed Date: 1996-08-20 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 551-7 Citation Subset: IM |
Affiliation:
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Department MBW, Biochemistry, Limburgs Universitair Centrum, Diepenbeek, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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5'-Nucleotidase
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antagonists & inhibitors,
metabolism Acid Anhydride Hydrolases / antagonists & inhibitors, metabolism Adenosine / pharmacology Adenosine Diphosphate / analogs & derivatives, pharmacology Adenosine Triphosphate / pharmacology Alkaline Phosphatase / antagonists & inhibitors, metabolism Animals Cell Division / drug effects* Cell Line Cell Line, Transformed Dinucleoside Phosphates / pharmacology Dipyridamole / pharmacology Enzyme Inhibitors / pharmacology Hela Cells Humans Levamisole / pharmacology Nucleoside-Triphosphatase Nucleosides / pharmacology* Nucleotides / pharmacology* Suramin / pharmacology Thymidine / metabolism Triazines / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Dinucleoside Phosphates; 0/Enzyme Inhibitors; 0/Nucleosides; 0/Nucleotides; 0/Triazines; 12236-82-7/Cibacron Blue F 3GA; 145-63-1/Suramin; 14769-73-4/Levamisole; 3768-14-7/adenosine 5'-methylenediphosphate; 50-89-5/Thymidine; 50304-44-4/P(1),P(5)-di(adenosine-5'-)pentaphosphate; 5542-28-9/diadenosine tetraphosphate; 56-65-5/Adenosine Triphosphate; 58-32-2/Dipyridamole; 58-61-7/Adenosine; 58-64-0/Adenosine Diphosphate; EC 3.1.3.1/Alkaline Phosphatase; EC 3.1.3.5/5'-Nucleotidase; EC 3.6.-/Acid Anhydride Hydrolases; EC 3.6.1.15/Nucleoside-Triphosphatase |
| Comments/Corrections | |
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