| Regulation and processing of a precursor form of eosinophil granule major basic protein (ProMBP) in differentiating eosinophils. | |
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MedLine Citation:
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PMID: 9657764 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cDNA for eosinophil granule major basic protein (MBP) encodes a prepromolecule with a total length of 222 amino acids (preproMBP). PreproMBP includes a secretory leader of 15 amino acids, an acidic propiece of 90 amino acids, and a basic MBP portion of 117 amino acids. The function of the propiece, which has a predicted pI of 3.9, is unknown, but it gives proMBP an overall acidic charge. Because proMBP is not found in mature eosinophils, we analyzed eosinophil differentiation in interleukin-5 (IL-5)-stimulated umbilical cord stem cells cultured for 24 days. By immunofluorescence, proMBP appeared by day 6 and peaked on day 18, whereas MBP was prominent at days 12 to 24. By day 6, Western blots detected heterogeneous glycosylated 33-kD proMBP; its peak expression occurred on day 12. Western blots showed sequential processing of 33-kD proMBP to an 18-kD intermediate form and finally to 14-kD MBP. By dual label immunoelectron microscopy, proMBP was localized primarily to large uncondensed eosinophil granules, whereas MBP was localized to granules containing a condensed central area. Thus, proMBP is likely expressed and processed as the granule condenses in a multistep process to 14-kD MBP in differentiating eosinophils. |
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Authors:
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P Popken-Harris; J Checkel; D Loegering; B Madden; M Springett; G Kephart; G J Gleich |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Blood Volume: 92 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 1998 Jul |
Date Detail:
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Created Date: 1998-08-06 Completed Date: 1998-08-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 623-31 Citation Subset: AIM; IM |
Affiliation:
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Department of Immunology, Mayo Clinic and Mayo Foundation, Rochester, MN 55905, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Blood Proteins / biosynthesis*, genetics* Cell Differentiation DNA, Complementary / analysis, genetics Eosinophil Granule Proteins Eosinophils / cytology, metabolism* Fetal Blood Gene Expression Regulation, Developmental Humans Microscopy, Immunoelectron Molecular Sequence Data Protein Precursors / biosynthesis, genetics Recombinant Proteins / biosynthesis, genetics Ribonucleases* |
| Grant Support | |
ID/Acronym/Agency:
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AI 07047/AI/NIAID NIH HHS; AI 09728/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Proteins; 0/DNA, Complementary; 0/Eosinophil Granule Proteins; 0/Protein Precursors; 0/Recombinant Proteins; EC 3.1.-/Ribonucleases |
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