Document Detail


Regulation of potassium fluxes in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  2245208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the regulation of K+ fluxes in Saccharomyces cerevisiae the dependence of K+ efflux and Rb+ influx on [K+]i, pHi, [Na+]i, membrane potential, cell volume, and turgor pressure were studied in cells with different K+ contents. By decreasing the cell volume with osmotic shocks and the cellular pH with butyric acid the following was found. (1) The K+ efflux induced by uncouplers decreases simultaneously with the decrease of the K+ content of the cell, but the process was insensitive to [K+]i, pHi, cell volume and turgor pressure. The internal presence of Na+ inhibited this K+ efflux. (2) The increase of the Vmax of Rb+ influx observed in low-K+ cells is due to the decrease of the pHi and probably mediated by the increase of the activity of the plasma membrane ATPase. The Vmax is independent of [K+]i, [Na+]i, cell volume and turgor pressure. (3) The decrease in the Km of Bt+ influx observed in low-K+ cells does not depend directly on [K+]i, pHi, cell volume or turgor pressure. If Na+ is present, [Na+]i might be directly involved in the regulation of the Km.
Authors:
J Ramos; R Haro; A Rodríguez-Navarro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1029     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-01-10     Completed Date:  1991-01-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  211-7     Citation Subset:  IM    
Affiliation:
Departamento de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos, Córdoba, Spain.
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MeSH Terms
Descriptor/Qualifier:
Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology
Hydrogen-Ion Concentration
Mutation
Potassium / metabolism*
Rubidium / pharmacokinetics
Saccharomyces cerevisiae / metabolism*
Chemical
Reg. No./Substance:
555-60-2/Carbonyl Cyanide m-Chlorophenyl Hydrazone; 7440-09-7/Potassium; 7440-17-7/Rubidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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