Document Detail


Regulation of photoreceptor Per1 and Per2 by light, dopamine and a circadian clock.
MedLine Citation:
PMID:  15245489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the Xenopus laevis retina, a principal model for retinal circadian organization, photoreceptors have all the properties of circadian oscillators. However, rhythmic oscillations of Per1 gene expression in the inner retina (but not photoreceptors) have been reported in mice with the suggestion that mice and frogs have a different retinal circadian organization. Although it is known that two period genes (xPer1 and xPer2) exhibit different temporal patterns of expression in the Xenopus retina, and that one (xPer2) is directly responsive to light and dopamine, it is not known whether this reflects the properties of period genes within photoreceptor oscillators or among distinct retinal cell populations. We addressed this by determining the cellular site of light and dopamine regulated xPer2 expression, and the diurnal expression of both xPer1 and xPer2 using in situ hybridization. Our data show that both xPer1 and xPer2 are expressed in most cell types in the retina, including inner nuclear neurons and ganglion cells. However, light and quinpirole, a dopamine agonist, increase xPer2 levels specifically in photoreceptors, and the effect of quinpirole, but not light, is blocked by pCPT-cAMP. Furthermore, antiphasic diurnal expression of xPer1 and xPer2 also occurs in photoreceptors. Our analysis does not provide insight into the near constitutive expression of period genes in the inner retina, but supports a model in which light- and dopamine regulated-xPer2 and rhythmic xPer1 play critical roles in entrainment and circadian oscillations within photoreceptors.
Authors:
Joseph C Besharse; Minhong Zhuang; Katie Freeman; Joseph Fogerty
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The European journal of neuroscience     Volume:  20     ISSN:  0953-816X     ISO Abbreviation:  Eur. J. Neurosci.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-07-12     Completed Date:  2004-09-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8918110     Medline TA:  Eur J Neurosci     Country:  France    
Other Details:
Languages:  eng     Pagination:  167-74     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA. jbeshars@mcw.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Northern / methods
Cell Count / methods
Circadian Rhythm / physiology*
Cyclic AMP / analogs & derivatives*,  pharmacology
Dopamine / pharmacology*
Dopamine Agonists / pharmacology
Enzyme Inhibitors / pharmacology
Eye
Eye Proteins / metabolism*
Gene Expression Regulation / drug effects*,  radiation effects
In Situ Hybridization
Light*
Male
Period Circadian Proteins
Photoreceptor Cells, Vertebrate* / drug effects,  radiation effects
Quinpirole / pharmacology
RNA, Messenger / metabolism
Retina / cytology,  metabolism
Thionucleotides / pharmacology
Xenopus Proteins
Xenopus laevis
Grant Support
ID/Acronym/Agency:
EY02414/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Dopamine Agonists; 0/Enzyme Inhibitors; 0/Eye Proteins; 0/Per1 protein, Xenopus; 0/Per1 protein, mouse; 0/Per2 protein, Xenopus; 0/Period Circadian Proteins; 0/RNA, Messenger; 0/Thionucleotides; 0/Xenopus Proteins; 41941-66-6/8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; 60-92-4/Cyclic AMP; 85760-74-3/Quinpirole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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