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Regulation of phosphoenolpyruvate carboxykinase and tyrosine transaminase in hepatoma cell cultures. 3. Comparative studies in H35, HTC, MH1C1 and RLC cells.
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MedLine Citation:
PMID:  4148931     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of N(6), O(2)'-dibutyryl cyclic AMP (DBcAMP) to regulate a number of metabolic events in four lines of cultured rat hepatomas has been examined. Although dexamethasone induces tyrosine transaminase in all four lines, DBcAMP induces this enzyme normally only in H35 cells. A slight increase in transaminase activity was seen with MH(1)C(1) cells and HTC cells, but no effect was detectable in RLC cells. In contrast, phosphoenolpyruvate carboxykinase activity is increased by both agents in H35 and MH(1)C(1) cells, but neither had any effect in HTC or RLC cells. DBcAMP caused a rapid inhibition of the growth rate and DNA synthesis and an increase in protein content in both H35 and MH(1)C(1) cells but not in HTC or RLC cells. The effect of DBcAMP on DNA synthesis in MH(1)C(1) cells could be reversed by deoxycytidine as is also the case with H35 cells. The resistance of HTC and RLC cells to DBcAMP was not due to reduced uptake or deacylation as judged by studies with [(3)H]DBcAMP. The cyclic nucleotide appears to enter the cells by passive diffusion as the intracellular concentration approaches that in the medium within 30-60 min. Possible explanations for the differential responses observed are discussed.
Authors:
H van Rijn; M M Bevers; R van Wijk; W D Wicks
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  60     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1974 Jan 
Date Detail:
Created Date:  1974-02-27     Completed Date:  1974-02-27     Revised Date:  2013-06-12    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  181-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Bucladesine / metabolism,  pharmacology
Carcinoma, Hepatocellular / enzymology*
Cell Count
Cell Division / drug effects
Cell Line
DNA / biosynthesis
Deoxyadenosines / metabolism
Deoxycytidine / pharmacology
Dexamethasone / pharmacology
Diffusion
Drug Resistance
Liver / drug effects,  enzymology
Liver Neoplasms
Neoplasm Proteins / biosynthesis
Phosphoenolpyruvate Carboxykinase (GTP) / metabolism*
Rats
Time Factors
Tritium
Tyrosine Transaminase / metabolism*
Chemical
Reg. No./Substance:
0/Deoxyadenosines; 0/Neoplasm Proteins; 10028-17-8/Tritium; 362-74-3/Bucladesine; 50-02-2/Dexamethasone; 9007-49-2/DNA; 951-77-9/Deoxycytidine; EC 2.6.1.5/Tyrosine Transaminase; EC 4.1.1.32/Phosphoenolpyruvate Carboxykinase (GTP)
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1974 by The Rockefeller University Press
Received Day: 7 Month: 5 Year: 1973
Revision Received Day: 28 Month: 9 Year: 1973
Print publication date: Day: 1 Month: 1 Year: 1974
Volume: 60 Issue: 1
First Page: 181 Last Page: 191
ID: 2109136
PubMed Id: 4148931

REGULATION OF PHOSPHOENOLPYRUVATE CARBOXYKINASE AND TYROSINE TRANSAMINASE IN HEPATOMA CELL CULTURES : III. Comparative Studies in H35, HTC, MH1C1, and RLC Cells
Han van Rijn
Marinus M. Bevers
Roeland van Wijk
Wesley D. Wicks
From the Van't Hoff Laboratory, State University, Utrecht, The Netherlands, and the Department of Pharmacology, University of Colorado Medical Center, Denver, Colorado 80220


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