| Regulation of phosphoenolpyruvate carboxykinase and tyrosine transaminase in hepatoma cell cultures. 3. Comparative studies in H35, HTC, MH1C1 and RLC cells. | |
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MedLine Citation:
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PMID: 4148931 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability of N(6), O(2)'-dibutyryl cyclic AMP (DBcAMP) to regulate a number of metabolic events in four lines of cultured rat hepatomas has been examined. Although dexamethasone induces tyrosine transaminase in all four lines, DBcAMP induces this enzyme normally only in H35 cells. A slight increase in transaminase activity was seen with MH(1)C(1) cells and HTC cells, but no effect was detectable in RLC cells. In contrast, phosphoenolpyruvate carboxykinase activity is increased by both agents in H35 and MH(1)C(1) cells, but neither had any effect in HTC or RLC cells. DBcAMP caused a rapid inhibition of the growth rate and DNA synthesis and an increase in protein content in both H35 and MH(1)C(1) cells but not in HTC or RLC cells. The effect of DBcAMP on DNA synthesis in MH(1)C(1) cells could be reversed by deoxycytidine as is also the case with H35 cells. The resistance of HTC and RLC cells to DBcAMP was not due to reduced uptake or deacylation as judged by studies with [(3)H]DBcAMP. The cyclic nucleotide appears to enter the cells by passive diffusion as the intracellular concentration approaches that in the medium within 30-60 min. Possible explanations for the differential responses observed are discussed. |
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Authors:
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H van Rijn; M M Bevers; R van Wijk; W D Wicks |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: The Journal of cell biology Volume: 60 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1974 Jan |
Date Detail:
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Created Date: 1974-02-27 Completed Date: 1974-02-27 Revised Date: 2013-03-26 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 181-91 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Bucladesine / metabolism, pharmacology Carcinoma, Hepatocellular / enzymology* Cell Count Cell Division / drug effects Cell Line DNA / biosynthesis Deoxyadenosines / metabolism Deoxycytidine / pharmacology Dexamethasone / pharmacology Diffusion Drug Resistance Liver / drug effects, enzymology Liver Neoplasms Neoplasm Proteins / biosynthesis Phosphoenolpyruvate Carboxykinase (GTP) / metabolism* Rats Time Factors Tritium Tyrosine Transaminase / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Deoxyadenosines; 0/Neoplasm Proteins; 10028-17-8/Tritium; 362-74-3/Bucladesine; 50-02-2/Dexamethasone; 9007-49-2/DNA; 951-77-9/Deoxycytidine; EC 2.6.1.5/Tyrosine Transaminase; EC 4.1.1.32/Phosphoenolpyruvate Carboxykinase (GTP) |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © 1974 by The Rockefeller University Press Received Day: 7 Month: 5 Year: 1973 Revision Received Day: 28 Month: 9 Year: 1973 Print publication date: Day: 1 Month: 1 Year: 1974 Volume: 60 Issue: 1 First Page: 181 Last Page: 191 ID: 2109136 PubMed Id: 4148931 |
| REGULATION OF PHOSPHOENOLPYRUVATE CARBOXYKINASE AND TYROSINE TRANSAMINASE IN HEPATOMA CELL CULTURES : III. Comparative Studies in H35, HTC, MH1C1, and RLC Cells | |
| Han van Rijn | |
| Marinus M. Bevers | |
| Roeland van Wijk | |
| Wesley D. Wicks | |
| From the Van't Hoff Laboratory, State University, Utrecht, The Netherlands, and the Department of Pharmacology, University of Colorado Medical Center, Denver, Colorado 80220 |
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