| Regulation of phosphatidylcholine biosynthesis in mammalian cells. I. Effects of phospholipase C treatment on phosphatidylcholine metabolism in Chinese hamster ovary cells and LM mouse fibroblasts. | |
| | |
MedLine Citation:
|
PMID: 6296084 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Addition of phospholipase C from Clostridium perfringens to cultures of Chinese hamster ovary (CHO) cells resulted in rapid degradation of cellular phosphatidylcholine with concomitant release of phosphocholine. The rate of incorporation of radiolabeled choline into lipids was increased 2-fold in phospholipase C-treated CHO cells as compared to untreated controls. The only enzyme in the pathway of phosphatidylcholine biosynthesis with increased activity in phospholipase C-treated cells was CTP:phosphocholine cytidylyltransferase, indicating that the cytidylyltransferase plays an important role in the stimulation of phosphatidylcholine biosynthesis. The phospholipase treatment was toxic to a CHO mutant cell line with abnormally low cytidylyltransferase activity. Mouse LM fibroblasts were resistant to enzymatic attack by phospholipase C, and cytidylyltransferase activity in LM cells did not change upon phospholipase C treatment. |
| | |
Authors:
|
R Sleight; C Kent |
Related Documents
:
|
22721864 - Reprogrammed cell delivery for personalized medicine. 9237774 - Survival, mutagenesis, and host cell reactivation in a chinese hamster ovary cell ercc1... 19291804 - Kinetics of cho a l mutant expression after treatment with gamma radiation, ems, and as... 4735454 - The effect of cell-to-cell contact on the surface morphology of chinese hamster ovary c... 23112854 - Metabolic profiling reveals sphingosine-1-phosphate kinase 2 and lyase as key targets o... 20014764 - Comparable susceptibilities of human 293 cells and insect tn-5b1-4 cells to photoactiva... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 258 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1983 Jan |
Date Detail:
|
Created Date: 1983-03-11 Completed Date: 1983-03-11 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 824-30 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line Choline-Phosphate Cytidylyltransferase Clostridium perfringens / enzymology Cricetinae Cricetulus Female Fibroblasts / metabolism Mice Nucleotidyltransferases / metabolism Ovary / metabolism* Phosphatidylcholines / biosynthesis* Phospholipases / metabolism* Tissue Distribution Type C Phospholipases / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
GM07211/GM/NIGMS NIH HHS; HD10580/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Phosphatidylcholines; EC 2.7.7.-/Nucleotidyltransferases; EC 2.7.7.15/Choline-Phosphate Cytidylyltransferase; EC 3.1.-/Phospholipases; EC 3.1.4.-/Type C Phospholipases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transformation by Rous sarcoma virus induces similar patterns of glycosaminoglycan synthesis in chic...
Next Document: Regulation of phosphatidylcholine biosynthesis in mammalian cells. II. Effects of phospholipase C tr...