Document Detail

Regulation of parathyroid cell gene expression in experimental uremia.
MedLine Citation:
PMID:  2104257     Owner:  NLM     Status:  MEDLINE    
The secondary hyperparathyroidism of renal failure is an important component of renal osteodystrophy. We studied PTHmRNA levels and their regulation in control and subtotal nephrectomized (5/6 NX) rats at 3 wk, as well as levels of the 1,25(OH)2D3 receptor mRNA in parathyroids. Serum 1,25(OH)2D levels were decreased in 5/6 NX, whereas PTHmRNA levels were increased (7 +/- 0.7 OD U, N = 4) compared to controls (2.1 +/- 1.2, P less than 0.01); both decreased after 1,25(OH)2D3 (100 pmol/100 g body weight). Similar results were found in 5/6 NX rats after 3 months. There was no change in actin mRNA levels. PTHmRNA levels were highest in 5/6 NX rats with the most severe renal failure. The parathyroid gland 1,25(OH)2D3 receptor mRNA levels were not different between 5/6 NX rats and controls and were not affected by 1,25(OH)2D3 (100 pmol/100 g body weight daily) at 1 or 3 days. PTHmRNA levels of 5/6 NX rats did not increase when the serum calcium was decreased from 2.8 +/- 0.05 mmol/L to 0.9 +/- 0.15 mmol/L at 3 or 5 h, which contrasted with the marked increase in PTHmRNA in normal rats after hypocalcemia. As in normal rats, after hypercalcemia (4.8 mmol/L at 1 h) there was no change in the 5/6 NX rats' PTHmRNA levels. These results show that 5/6 NX rats have increased PTHmRNA levels that are normally regulated by injected 1,25(OH)2D3 but not by calcium. Parathyroid gland 1,25(OH)2D receptor mRNA levels are not increased in 5/6 NX in contrast to the increased PTHmRNA, which reflects the larger glands of uremia. 1,25(OH)2D receptor mRNA levels were not regulated by 1,25(OH)2D3.(ABSTRACT TRUNCATED AT 250 WORDS)
Y Shvil; T Naveh-Many; P Barach; J Silver
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the American Society of Nephrology : JASN     Volume:  1     ISSN:  1046-6673     ISO Abbreviation:  J. Am. Soc. Nephrol.     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1991-10-21     Completed Date:  1991-10-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9013836     Medline TA:  J Am Soc Nephrol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  99-104     Citation Subset:  IM    
Department of Nephrology, Hadassah University Hospital, Jerusalem, Israel.
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MeSH Terms
Calcitriol / blood,  pharmacology
Calcium / blood,  pharmacology
Disease Models, Animal
Gene Expression Regulation / drug effects
Hyperparathyroidism, Secondary / etiology,  genetics,  metabolism
Parathyroid Glands / metabolism,  pathology
Parathyroid Hormone / genetics*
RNA, Messenger / genetics,  metabolism
Uremia / complications,  genetics*,  metabolism
Grant Support
Reg. No./Substance:
0/Parathyroid Hormone; 0/RNA, Messenger; 32222-06-3/Calcitriol; 7440-70-2/Calcium

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