Document Detail

Regulation of paracellular Na+ and Cl(-) conductances by hydrostatic pressure.
MedLine Citation:
PMID:  19524694     Owner:  NLM     Status:  MEDLINE    
The effect of hydrostatic pressure on the paracellular ion conductance (Gp) composed of the Na(+) conductance (G(Na)) and the Cl(-) conductance (G(Cl)) has been Investigated. Gp, G(Na) and G(Cl) were time-dependently increased after applying an osmotic gradient generated by NaCl with basolateral hypotonicity. Hydrostatic pressure (1-4cm H2O) applied from the basolateral side enhanced the osmotic gradient-induced increase in Gp, G(Na) and G(Cl) in a magnitude-dependent manner, while the hydrostatic pressure applied from the apical side diminished the osmotic gradient-induced increase in Gp, G(Na) and G(Cl). How the hydrostatic pressure influences Gp, G(Na) and G(Cl) under an isosmotic condition was also investigated. Gp, G(Na) and G(Cl) were stably constant under a condition with basolateral application of sucrose canceling the NaCl-generated osmotic gradient (an isotonic condition). Even under this stable condition, the basolaterally applied hydrostatic pressure drastically elevated Gp, G(Na) and G(Cl), while apically applied hydrostatic pressure had little effect on Gp, G(Na) or G(Cl). Taken together, these observations suggest that certain factors controlled by the basolateral osmolality and the basolaterally applied hydrostatic pressure mainly regulate the Gp, G(Na) and G(Cl).
Shinsaku Tokuda; Naomi Niisato; Toshiki Nagai; Akiyuki Taruno; Ken-Ichi Nakajima; Hiroaki Miyazaki; Toshiki Yamada; Shigekuni Hosogi; Mariko Ohta; Kyosuke Nishio; Yoshinobu Iwasaki; Yoshinori Marunaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-12
Journal Detail:
Title:  Cell biology international     Volume:  33     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-31     Completed Date:  2009-12-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  949-56     Citation Subset:  IM    
Department of Molecular Cell Physiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
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MeSH Terms
Cell Line
Chlorides / metabolism*
Hydrostatic Pressure*
Osmotic Pressure / physiology
Sodium / metabolism*
Sodium Chloride / metabolism*
Tight Junctions / metabolism,  physiology*
Xenopus laevis
Reg. No./Substance:
0/Chlorides; 7440-23-5/Sodium; 7647-14-5/Sodium Chloride

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