Document Detail

Regulation of p21, MMP-1, and MDR-1 Expression in Human Colon Carcinoma HT29 Cells by Tian Xian Liquid, a Chinese Medicinal Formula, In Vitro and In Vivo.
MedLine Citation:
PMID:  20702488     Owner:  NLM     Status:  In-Data-Review    
Ethnopharmacological relevance. Tian-Xian liquid (TXL), a commercially available Chinese medicine decoction, has been used as an anticancer dietary agent for more than 10 years without reported side effects. Aim of the study. The safety and quality consistency of TXL and its mechanisms of action on antiproliferation, antimetastasis, and reversion of multidrug resistance (MDR) regimens were explored. Materials and methods. In this study, an atomic absorption spectrophotometer and reversed phase high performance liquid chromatography with photodiode array detection (HPLC-DAD) were used to evaluate the main toxic elements and the quality consistency among different batches of TXL extracts, respectively. HT29 human colon cancer cell line and tumor-bearing nude mice were used. TXL was provided by China-Japan Feida Union Company Limited. The effect of TXL on in vitro proliferation of HT29 human colon cancer cell line was examined. The percentages of treated cells distributed in different phases of the cell cycles were analyzed by flow cytometry. Antiproliferative effect after treatment with TXL was assessed by determination of the protein levels of p21, cyclinD1, PCNA, and cdk-2, which are the key regulators for cell cycle progression. Meanwhile, the protein levels of MMP-1 and MDR-1 (multidrug resistance protein-1) were also determined to assess the effect of TXL on antimetastasis and reversion of MDR regimen, respectively. RESULTS: The contents of main toxic elements were lower in TXL extract compared with the standard set by the Department of Health of the Government of Hong Kong Special Administrative Region (SAR). Our HPLC results showed that the relative standard deviations of the amount of the 5 standards were less than 5% in different batches of TXL. Immunoblotting analysis revealed a dramatic induction of cyclin kinase inhibitor p21 as well as an inhibition of cyclinD1, PCNA, and cdk-2 in the TXL-treated in vitro models, thereby, impeding cell progression from G1/S phase. Results obtained from the in vivo study also demonstrated that TXL upregulated the protein level of p21 and downregulated the protein levels of MMP-1 and MDR-1. CONCLUSIONS: Results obtained from the present investigation not only demonstrate the safety and quality of TXL extract but also demonstrate that TXL possesses antiproliferative and antimetastatic activities and brings about reversion of MDR on HT29 cell and on xenografted tissue in tumor-implanted nude mice.
Stephen C W Sze; Kam L Wong; Wing K Liu; Tzi B Ng; Jack H Wong; Ho P Cheung; Christine M L Yow; Ellie S M Chu; Qing Liu; Yong M Hu; Kam W Tsang; Wai S Lee; Yao Tong
Related Documents :
1969768 - Novel screening method for agents that overcome classical multidrug resistance in a hum...
7986198 - Comparison of mechanisms responsible for resistance to idarubicin and daunorubicin in m...
15167518 - Limitations of (99m)tc tetrofosmin in assessing reversal effects of verapamil on the fu...
17092838 - Establishment of subcellular fractionation techniques to monitor the intracellular fate...
384568 - A simple method for decreasing the toxicity of polyethylene glycol in mammalian cell hy...
21220508 - Set8 is degraded via pcna-coupled crl4(cdt2) ubiquitylation in s phase and after uv irr...
Publication Detail:
Type:  Journal Article     Date:  2010-08-11
Journal Detail:
Title:  Integrative cancer therapies     Volume:  10     ISSN:  1552-695X     ISO Abbreviation:  Integr Cancer Ther     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-04-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128834     Medline TA:  Integr Cancer Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  58-69     Citation Subset:  IM    
School of Chinese Medicine, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  An experimental study on the antileukemia effects of gypenosides in vitro and in vivo.
Next Document:  Cancer chemoprevention by 7-prenyloxycoumarins: a role for 5-lipoxygenase inhibition?