| Regulation of ovarian steroidogenesis in vitro by IGF-I and insulin in common carp, Cyprinus carpio: stimulation of aromatase activity and P450arom gene expression. | |
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MedLine Citation:
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PMID: 19897011 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Regulation of ovarian steroidogenesis in vitro by recombinant human insulin-like growth factor-I (IGF-I) and bovine insulin (b-insulin) was investigated in intact follicles and isolated follicular cells of carp, Cyprinus carpio at vitellogenic stage of oocyte maturation. In intact follicles, IGF-I and b-insulin stimulated testosterone and 17beta-estradiol production in vitro. In isolated theca cells, IGF-I and b-insulin stimulated testosterone production, whereas in granulosa cells, they stimulated 17beta-estradiol production when testosterone was added in the incubation medium as precursor substrate. In intact follicles and in theca cells, IGF-I and b-insulin had no effect on HCG-stimulated testosterone production. HCG-stimulated 17beta-estradiol production, however, was significantly increased by IGF-I and b-insulin. To clarify the mechanism of 17beta-estradiol production by the ovarian follicles during vitellogenic stage of carp, effects of IGF-I and b-insulin either alone or in combination with HCG on aromatase activity (conversion of testosterone to 17beta-estradiol) and cytochrome P450 aromatase (P450arom) gene expression were investigated in vitro. IGF-I and b-insulin alone stimulated aromatase activity and P450arom gene expression and significantly enhanced HCG-induced enzyme activity and P450arom gene expression. Our results thus indicate that IGF-I and b-insulin alone can stimulate testosterone and 17beta-estradiol production in vitellogenic follicles of C. carpio by stimulating aromatase activity and P450arom gene expression. Evidence also provided for the modulation of HCG-induced aromatase activity and P450arom gene expression by IGF-I and b-insulin in such follicles. |
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Authors:
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Sudipta Paul; Kousik Pramanick; Sourav Kundu; Dhyanendra Kumar; Dilip Mukherjee |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-06 |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: 315 ISSN: 1872-8057 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-07-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: Ireland |
Other Details:
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Languages: eng Pagination: 95-103 Citation Subset: IM |
Copyright Information:
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2009 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Endocrinology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aromatase* / genetics, metabolism Carps* / anatomy & histology, metabolism Cattle Chorionic Gonadotropin / pharmacology Dose-Response Relationship, Drug Estradiol / metabolism* Female Gene Expression Regulation / drug effects Humans Insulin / pharmacology* Insulin-Like Growth Factor I / pharmacology* Ovary* / drug effects, enzymology, physiology Reproductive Control Agents / pharmacology Testosterone / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 0/Reproductive Control Agents; 11061-68-0/Insulin; 50-28-2/Estradiol; 58-22-0/Testosterone; 67763-96-6/Insulin-Like Growth Factor I; EC 1.14.14.1/Aromatase |
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