| Regulation of nuclear receptor and cofactor expression in breast cancer cell lines. | |
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MedLine Citation:
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PMID: 12656669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The aim of this study was to compare the expression profile of nuclear receptors (NRs) and cofactors in different breast cancer cell lines as well as their regulation by estradiol, insulin and progestin R5020. METHODS: Expression of NRs and cofactors were determined from MCF-7, T-47D and ZR-75-1 breast cancer cell lines. Multiprobe ribonuclease protection assay and real-time RT-PCR were used to quantitate mRNA levels of steroid receptors, vitamin D receptors (VDR) and retinoic acid receptors (RAR) and cofactors: amplified in breast cancer-1, cyclic AMP response element binding protein (CBP), p300/CBP-associated factor, p300, nuclear receptor corepressor and silencing mediator of repressed transcription. RESULTS: Basal expression levels of NRs and cofactors varied depending on the cell line. Cell line-specific regulation of androgen receptor, estrogen receptor-alpha (ERalpha), RARalpha, RARgamma and VDR expression was observed after estradiol treatment. Likewise, differences in the regulation of ERalpha, RARalpha and VDR expression after R5020 treatment were observed. We did not observe significant regulation of cofactor expression after estradiol, insulin or progestin treatment in any cell line analyzed. CONCLUSIONS: The results showed that not only is the expression profile of the NRs and cofactors cell line specific but also the regulation of NR expression. Thus the determinants of the ligand action (receptor and cofactor expression) varied considerably among different cell clones of the breast cancer cells. This suggested a gradient of NR-ligand sensitivities in the hormone-dependent breast cancers, which produces an additional challenge in developing novel ligands for hormone replacement therapy and breast cancer treatment. |
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Authors:
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Annika Vienonen; Susanna Miettinen; Tommi Manninen; Lucia Altucci; Emmanuelle Wilhelm; Timo Ylikomi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of endocrinology / European Federation of Endocrine Societies Volume: 148 ISSN: 0804-4643 ISO Abbreviation: Eur. J. Endocrinol. Publication Date: 2003 Apr |
Date Detail:
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Created Date: 2003-03-26 Completed Date: 2003-05-15 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9423848 Medline TA: Eur J Endocrinol Country: England |
Other Details:
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Languages: eng Pagination: 469-79 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Medical School, University of Tampere, FIN-33014 Tampere, Finland. annika.vienonen@uta.fi |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetyltransferases
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genetics Breast Neoplasms / metabolism* Cell Cycle Proteins / genetics Cyclic AMP Response Element-Binding Protein / genetics Estradiol / pharmacology Gene Expression Regulation, Neoplastic* / drug effects Histone Acetyltransferases Humans Insulin / pharmacology Nuclear Proteins / genetics Nuclear Receptor Co-Repressor 1 Promegestone / pharmacology RNA, Messenger / analysis Receptors, Calcitriol / genetics Receptors, Cytoplasmic and Nuclear / genetics* Receptors, Retinoic Acid / genetics Receptors, Steroid / genetics Repressor Proteins / genetics Reverse Transcriptase Polymerase Chain Reaction Silencer Elements, Transcriptional Transcription Factors Tumor Cells, Cultured p300-CBP Transcription Factors |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Cyclic AMP Response Element-Binding Protein; 0/NCOR1 protein, human; 0/Nuclear Proteins; 0/Nuclear Receptor Co-Repressor 1; 0/RNA, Messenger; 0/Receptors, Calcitriol; 0/Receptors, Cytoplasmic and Nuclear; 0/Receptors, Retinoic Acid; 0/Receptors, Steroid; 0/Repressor Proteins; 0/Transcription Factors; 11061-68-0/Insulin; 34184-77-5/Promegestone; 50-28-2/Estradiol; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.48/Histone Acetyltransferases; EC 2.3.1.48/p300-CBP Transcription Factors; EC 2.3.1.48/p300-CBP-associated factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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