Document Detail

Regulation of the nuclear-coded peptides of yeast cytochrome c oxidase.
MedLine Citation:
PMID:  6280749     Owner:  NLM     Status:  MEDLINE    
We have analyzed the catabolite regulation of cytochrome oxidase by assaying changes in the synthesis of precursors of the nuclear-coded peptides (IV--VII) of cytochrome c oxidase in an in vitro reticulocyte cell-free system programmed with RNA isolated from cells grown in either glucose or raffinose. As a first step, we have characterized antibodies which bind to the precursors of subunits V and VI. Initial translation products for subunits IV and VII have also been tentatively identified by utilizing these antibodies. The messenger RNAs coding for the precursors of the nuclear-coded subunits fall in the expected size range of 8--15 S. Catabolite repression of the nuclear-coded oxidase peptides appears to be regulated by the abundance of their messenger RNAs. Translation of messenger RNA isolated from yeast cells grown on glucose indicates a coordinate and uniform increase in precursor synthesis during glucose derepression. In contrast, when RNA isolated from raffinose (derepressed) grown cells is used to direct cell-free translation, precursor abundance is high throughout growth, although the synthesis of some of the species changes in a complex pattern of ratio and abundance. These data indicate that the abundance of the messengers for the nuclear-coded precursors is regulated in a fashion dependent on the physiologic state of the cell.
A Lustig; G Padmanaban; M Rabinowitz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  21     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1982 Jan 
Date Detail:
Created Date:  1982-07-19     Completed Date:  1982-07-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  309-16     Citation Subset:  IM    
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MeSH Terms
Electron Transport Complex IV / genetics*
Enzyme Precursors / genetics
Enzyme Repression
Gene Expression Regulation
Intracellular Membranes / enzymology
Macromolecular Substances
Mitochondria / enzymology
Molecular Weight
RNA, Messenger / metabolism
Saccharomyces cerevisiae / enzymology,  genetics*
Transcription, Genetic
Grant Support
Reg. No./Substance:
0/Enzyme Precursors; 0/Macromolecular Substances; 0/RNA, Messenger; EC Transport Complex IV

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