| Regulation of the nuclear-coded peptides of yeast cytochrome c oxidase. | |
| | |
MedLine Citation:
|
PMID: 6280749 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have analyzed the catabolite regulation of cytochrome oxidase by assaying changes in the synthesis of precursors of the nuclear-coded peptides (IV--VII) of cytochrome c oxidase in an in vitro reticulocyte cell-free system programmed with RNA isolated from cells grown in either glucose or raffinose. As a first step, we have characterized antibodies which bind to the precursors of subunits V and VI. Initial translation products for subunits IV and VII have also been tentatively identified by utilizing these antibodies. The messenger RNAs coding for the precursors of the nuclear-coded subunits fall in the expected size range of 8--15 S. Catabolite repression of the nuclear-coded oxidase peptides appears to be regulated by the abundance of their messenger RNAs. Translation of messenger RNA isolated from yeast cells grown on glucose indicates a coordinate and uniform increase in precursor synthesis during glucose derepression. In contrast, when RNA isolated from raffinose (derepressed) grown cells is used to direct cell-free translation, precursor abundance is high throughout growth, although the synthesis of some of the species changes in a complex pattern of ratio and abundance. These data indicate that the abundance of the messengers for the nuclear-coded precursors is regulated in a fashion dependent on the physiologic state of the cell. |
| | |
Authors:
|
A Lustig; G Padmanaban; M Rabinowitz |
Related Documents
:
|
21290459 - (1) h-mrs can detect aberrant glycosylation in tumour cells: a study of the hela cell l... 11568499 - Clinical role of polyamine analysis: problem and promise. 11539049 - Separation of mouse testis cells on a celsep (tm) apparatus and their usefulness as a s... 5500309 - The use of conventional and zonal centrifugation to study the life cycle of mammalian c... 19206549 - Inducible rna interference-mediated gene silencing using nanostructured gene delivery a... 2702649 - Antitumor effect of a new multienzyme inhibitor of polyamine synthetic pathway, methylg... 1868629 - Drug sensitivity and metastatic ability in b16 melanoma cells. 16107889 - Translational study of vitamin d differentiation therapy of myeloid leukemia: effects o... 10340789 - Expression of stress-response protein 60 in lens epithelial cells in atopic cataract. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biochemistry Volume: 21 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1982 Jan |
Date Detail:
|
Created Date: 1982-07-19 Completed Date: 1982-07-19 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 309-16 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Electron Transport Complex IV
/
genetics* Enzyme Precursors / genetics Enzyme Repression Gene Expression Regulation Genes Intracellular Membranes / enzymology Macromolecular Substances Mitochondria / enzymology Molecular Weight RNA, Messenger / metabolism Saccharomyces cerevisiae / enzymology, genetics* Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
|
HL-04442/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Enzyme Precursors; 0/Macromolecular Substances; 0/RNA, Messenger; EC 1.9.3.1/Electron Transport Complex IV |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Immune electron microscopy and a cultural test in the diagnosis of adenovirus ocular infection.
Next Document: Purification and characterization of the messenger ribonucleic acid capping enzyme GTP:RNA guanylylt...