Document Detail

Regulation of the murine renal vitamin D receptor by 1,25-dihydroxyvitamin D3 and calcium.
MedLine Citation:
PMID:  12900504     Owner:  NLM     Status:  MEDLINE    
Renal vitamin D receptor (VDR) is required for 1,25-dihydroxyvitamin D3-[1,25(OH)2D3]-induced renal reabsorption of calcium and for 1,25(OH)2D3-induced 1,25(OH)2D3 24-hydroxylase. The long-term effect of vitamin D and dietary calcium on the expression of renal VDR was examined in the nonobese diabetic mouse. Vitamin D-deficient and vitamin D-replete mice were maintained on diets containing 0.02%, 0.25%, 0.47%, and 1.20% calcium with or without 50 ng of 1,25(OH)2D3 per day. Vitamin D-replete mice on a 1.20% calcium diet had renal VDR levels of 165 fmol/mg protein. Calcium restriction caused renal VDR levels to decrease to <30 fmol/mg protein in vitamin D-deficient mice and to approximately 80 fmol/mg protein in vitamin D-replete mice. When dietary calcium was present, 50 ng of 1,25(OH)2D3 elevated the VDR levels 2- to 10-fold, depending on vitamin D status and the level of calcium. In the absence of either vitamin D or calcium, the VDR mRNA was expressed at a basal level. 1,25(OH)2D3 supplementation caused relative VDR mRNA to increase 8- to 10-fold in the vitamin D-deficient mouse when dietary calcium was available. This increase was completely absent in the calcium-restricted mice. This in vivo study demonstrates that 1,25(OH)2D3 and calcium are both required for renal VDR mRNA expression above a basal level, furthering our understanding of the complex regulation of renal VDR by 1,25(OH)2D3 and calcium.
Kevin D Healy; Julia B Zella; Jean M Prahl; Hector F DeLuca
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-08-04
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  100     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-08-20     Completed Date:  2003-10-29     Revised Date:  2014-01-13    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9733-7     Citation Subset:  IM    
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MeSH Terms
25-Hydroxyvitamin D3 1-alpha-Hydroxylase / genetics,  metabolism
Base Sequence
Calcitriol / administration & dosage,  pharmacology*
Calcium, Dietary / administration & dosage,  pharmacology*
Cytochrome P-450 Enzyme System / genetics,  metabolism
DNA / genetics
Gene Expression Regulation / drug effects
Kidney / drug effects*,  metabolism*
Mice, Inbred NOD
RNA, Messenger / genetics,  metabolism
Receptors, Calcitriol / genetics*,  metabolism*
Steroid Hydroxylases / genetics,  metabolism
Reg. No./Substance:
0/Calcium, Dietary; 0/RNA, Messenger; 0/Receptors, Calcitriol; 9007-49-2/DNA; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.-/25-Hydroxyvitamin D3 1-alpha-Hydroxylase; EC 1.14.-/Steroid Hydroxylases; EC D 24-hydroxylase; FXC9231JVH/Calcitriol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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