| Regulation of the mouse protein targeting to glycogen (PTG) promoter by the FoxA2 forkhead protein and by 3',5'-cyclic adenosine 5'-monophosphate in H4IIE hepatoma cells. | |
| | |
MedLine Citation:
|
PMID: 16627590 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The scaffolding protein, protein targeting to glycogen (PTG), orchestrates the signaling of several metabolic enzymes involved in glycogen synthesis. However, little is known concerning the regulation of PTG itself. In this study, we have cloned and characterized the mouse promoter of PTG. We identified multiple FoxA2 binding sites within this region. FoxA2 is a member of the forkhead family of transcription factors that has recently been implicated in the cAMP-dependent regulation of several genes involved in liver metabolism. Using luciferase reporter constructs, we demonstrate that FoxA2 transactivates the PTG promoter in H4IIE hepatoma cells. Nuclear extracts prepared from mouse liver and H4IIE cells were able to bind a FoxA2-specific probe derived within the PTG promoter region. Chromatin immunoprecipitation experiments further demonstrate that FoxA2 binds to the PTG promoter in vivo. Finally, we show that treatment with cAMP analogs activates the PTG promoter and significantly increases PTG levels in H4IIE cells. Our results provide a framework to investigate how additional transcription factors may regulate PTG expression in other cell types. |
| | |
Authors:
|
Alan Cheng; Mei Zhang; Sean M Crosson; Zhao Q Bao; Alan R Saltiel |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-04-20 |
Journal Detail:
|
Title: Endocrinology Volume: 147 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 2006 Jul |
Date Detail:
|
Created Date: 2006-06-16 Completed Date: 2006-07-24 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
|
Languages: eng Pagination: 3606-12 Citation Subset: AIM; IM |
Affiliation:
|
Department of Internal Medicine, Life Sciences Institute, University of Michigan, Ann Arbor, 48109, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Base Sequence Carcinoma, Hepatocellular / metabolism* Cell Line, Tumor Gene Expression Regulation* Glycogen / genetics* Hepatocyte Nuclear Factor 3-beta / metabolism* Intracellular Signaling Peptides and Proteins / metabolism* Liver / metabolism Mice Molecular Sequence Data Muscles / metabolism Promoter Regions, Genetic* |
| Grant Support | |
ID/Acronym/Agency:
|
5R01DK060597/DK/NIDDK NIH HHS; F32DK064551/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Foxa2 protein, mouse; 0/Intracellular Signaling Peptides and Proteins; 0/Ppp1r3c protein, mouse; 135845-92-0/Hepatocyte Nuclear Factor 3-beta; 9005-79-2/Glycogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: RGS2 is regulated by angiotensin II and functions as a negative feedback of aldosterone production i...
Next Document: Amphotericin B tissue distribution in autopsy material after treatment with liposomal amphotericin B...