Document Detail

Regulation of the mitochondrial ATP-synthase in human fibroblasts.
MedLine Citation:
PMID:  7704949     Owner:  NLM     Status:  MEDLINE    
The F1F0-ATPase activity of mitochondrial complex V can be rapidly measured in sonicated preparations of monolayer skin fibroblast cultures from children. We show that direct regulation at the level of ATP-synthase occurs in these cell preparations. ATP-synthase capacity is decreased in response to blocking of the respiratory chain by cyanide (mimicking anoxia) or uncoupling of mitochondria. ATP-synthase capacity falls to 60% and 35% of control, respectively. Up-regulation of ATP-synthase can be demonstrated in fibroblasts exposed to 4 mmol/l calcium (127% of control). Mitochondrial recovery was unchanged under the different incubation conditions as judged by the activity of mitochondrial marker enzymes. We conclude that direct regulation at the level of ATP-synthase occurs in vivo in human fibroblasts. The naturally occurring inhibitor protein IF1 and the calcium binding inhibitor protein CaBI may be involved in this regulation of ATP-synthase.
A M Das; D J Byrd; J Brodehl
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  231     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1995-05-08     Completed Date:  1995-05-08     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  61-8     Citation Subset:  IM    
Department of Pediatric Nephrology and Metabolic Disorders, Children's Hospital, Hannover Medical School, Germany.
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MeSH Terms
Calcium / pharmacology
Cyanides / pharmacology
Fibroblasts / metabolism*,  ultrastructure
Infant, Newborn
Mitochondria / enzymology
Proton-Translocating ATPases / chemistry,  drug effects,  metabolism*
Skin / cytology
Reg. No./Substance:
0/Cyanides; 7440-70-2/Calcium; EC ATPases

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