Document Detail

Regulation of mitochondria distribution by RhoA and formins.
MedLine Citation:
PMID:  16434478     Owner:  NLM     Status:  MEDLINE    
The distribution of mitochondria is strictly controlled by the cell because of their vital role in energy supply, regulation of cytosolic Ca2+ concentration and apoptosis. We employed cultured mammalian CV-1 cells and Drosophila BG2-C2 neuronal cells with enhanced green fluorescent protein (EGFP)-tagged mitochondria to investigate the regulation of their movement and anchorage. We show here that lysophosphatidic acid (LPA) inhibits fast mitochondrial movements in CV-1 cells acting through the small GTPase RhoA. The action of RhoA is mediated by its downstream effectors: formin-homology family members mDia1 in mammalian cells and diaphanous in Drosophila. Overexpression of constitutively active mutant forms of formins leads to dramatic loss of mitochondrial motility and to their anchorage to actin microfilaments. Conversely, depletion of endogenous diaphanous protein in BG2-C2 cells by RNA interference (RNAi) stimulates the mitochondrial movement. These effects are not simply explained by increased cytoplasm viscosity resulting from an increased F-actin concentration since stimulators of Arp2/3-dependent actin polymerization and jasplakinolide do not cause inhibition. The observed effects are highly specific to mitochondria since perturbations of diaphanous or mDia1 have no effect on movement of other membrane organelles. Thus, mitochondrial movement is controlled by the small GTPase RhoA and this control is mediated by formins.
Alexander A Minin; Alexander V Kulik; Fatima K Gyoeva; Ying Li; Gohta Goshima; Vladimir I Gelfand
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-01-24
Journal Detail:
Title:  Journal of cell science     Volume:  119     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-09     Completed Date:  2006-06-12     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  659-70     Citation Subset:  IM    
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MeSH Terms
Calcium / metabolism
Carrier Proteins / metabolism
Cell Line
Microfilament Proteins / metabolism*
Microtubules / metabolism,  ultrastructure
Mitochondria / metabolism*,  ultrastructure
Neurons / metabolism,  ultrastructure
rhoA GTP-Binding Protein / metabolism*
Grant Support
Reg. No./Substance:
0/Carrier Proteins; 0/Diap1 protein, mouse; 0/Microfilament Proteins; EC GTP-Binding Protein; SY7Q814VUP/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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