Document Detail

Regulation of microfilament organization and anchorage-independent growth by tropomyosin 1.
MedLine Citation:
PMID:  8524798     Owner:  NLM     Status:  MEDLINE    
Variants of chemically immortalized Syrian hamster embryo cells that had either retained (supB+) or lost (supB-) the ability to suppress tumorigenicity when hybridized with a fibrosarcoma cell line were subcloned. Both supB cell types are nontumorigenic; however, the supB- but not supB+ cells exhibit conditional anchorage-independent growth. Alterations of actin microfilament organization were observed in supB- but not supB+ cells that corresponded to a significant reduction of the actin-binding protein tropomyosin 1 (TM-1) in subB- cells. To examine the possibility of a direct relationship between TM-1 expression and the subB- phenotype, subB+ cells were transfected with an expression vector containing the TM-1 cDNA in an antisense orientation. The antisense-induced reduction of TM-1 levels in supB+ clones caused a microfilament reorganization and conferred anchorage-independent growth potential that were indistinguishable from those characteristic of supB- cells. These data provide direct evidence that TM-1 regulates both microfilament organization and anchorage-independent growth and suggest that microfilament alterations are sufficient for anchorage-independent growth.
J Boyd; J I Risinger; R W Wiseman; B A Merrick; J K Selkirk; J C Barrett
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  92     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-01-24     Completed Date:  1996-01-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  11534-8     Citation Subset:  IM    
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA.
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MeSH Terms
Blotting, Northern
Cell Adhesion / genetics*
Cell Division
Cell Transformation, Neoplastic / genetics*
Cells, Cultured
Cloning, Molecular
Cytoskeleton / ultrastructure
DNA Probes
Drosophila Proteins*
Electrophoresis, Gel, Two-Dimensional
Embryo, Mammalian / cytology
Genes, Tumor Suppressor
Genetic Variation
Microfilaments / ultrastructure*
Microscopy, Fluorescence
Molecular Sequence Data
RNA, Antisense
Recombinant Proteins / biosynthesis
Tropomyosin / biosynthesis,  genetics*
Reg. No./Substance:
0/DNA Probes; 0/Drosophila Proteins; 0/RNA, Antisense; 0/Recombinant Proteins; 0/Tm2 protein, Drosophila; 0/Tropomyosin

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