Document Detail


Regulation of miR-200c by nuclear receptors PPARα, LRH-1 and SHP.
MedLine Citation:
PMID:  22100809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated regulation of miR-200c expression by nuclear receptors. Ectopic expression of miR-200c inhibited MHCC97H cell migration, which was abrogated by the synergistic effects of PPARα and LRH-1 siRNAs. The expression of miR-200c was decreased by PPARα/LRH-1 siRNAs and increased by SHP siRNAs, and overexpression of the receptors reversed the effects of their respective siRNAs. SHP siRNAs also drastically enhanced the ability of the LRH-1 agonist RJW100 to induce miR-200c and downregulate ZEB1 and ZEB2 proteins. Co-expression of PPARα and LRH-1 moderately transactivated the miR-200c promoter, which was repressed by SHP co-expression. RJW100 caused strong activation of the miR-200c promoter. This is the first report to demonstrate that miR-200c expression is controlled by nuclear receptors.
Authors:
Yuxia Zhang; Zhihong Yang; Richard Whitby; Li Wang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-11-11
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  416     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-13     Completed Date:  2012-01-27     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  135-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Department of Medicine, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Cell Movement / genetics
Gene Expression Regulation, Neoplastic*
Gene Knockdown Techniques
Humans
MicroRNAs / antagonists & inhibitors,  genetics*
PPAR alpha / genetics,  metabolism*
Promoter Regions, Genetic
RNA, Small Interfering / genetics
Receptors, Cytoplasmic and Nuclear / genetics,  metabolism*
Transcriptional Activation
Grant Support
ID/Acronym/Agency:
DK080440/DK/NIDDK NIH HHS; R01 DK080440/DK/NIDDK NIH HHS; R01 DK080440-05/DK/NIDDK NIH HHS; T32CA092347/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/MIRN200 microRNA, human; 0/MicroRNAs; 0/NR5A2 protein, human; 0/PPAR alpha; 0/RNA, Small Interfering; 0/Receptors, Cytoplasmic and Nuclear; 0/nuclear receptor subfamily 0, group B, member 2
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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