| Regulation of miR-200c by nuclear receptors PPARα, LRH-1 and SHP. | |
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MedLine Citation:
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PMID: 22100809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We investigated regulation of miR-200c expression by nuclear receptors. Ectopic expression of miR-200c inhibited MHCC97H cell migration, which was abrogated by the synergistic effects of PPARα and LRH-1 siRNAs. The expression of miR-200c was decreased by PPARα/LRH-1 siRNAs and increased by SHP siRNAs, and overexpression of the receptors reversed the effects of their respective siRNAs. SHP siRNAs also drastically enhanced the ability of the LRH-1 agonist RJW100 to induce miR-200c and downregulate ZEB1 and ZEB2 proteins. Co-expression of PPARα and LRH-1 moderately transactivated the miR-200c promoter, which was repressed by SHP co-expression. RJW100 caused strong activation of the miR-200c promoter. This is the first report to demonstrate that miR-200c expression is controlled by nuclear receptors. |
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Authors:
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Yuxia Zhang; Zhihong Yang; Richard Whitby; Li Wang |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-11-11 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 416 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-13 Completed Date: 2012-01-27 Revised Date: 2013-05-23 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 135-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Medicine, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT 84132, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Tumor Cell Movement / genetics Gene Expression Regulation, Neoplastic* Gene Knockdown Techniques Humans MicroRNAs / antagonists & inhibitors, genetics* PPAR alpha / genetics, metabolism* Promoter Regions, Genetic RNA, Small Interfering / genetics Receptors, Cytoplasmic and Nuclear / genetics, metabolism* Transcriptional Activation |
| Grant Support | |
ID/Acronym/Agency:
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DK080440/DK/NIDDK NIH HHS; R01 DK080440/DK/NIDDK NIH HHS; R01 DK080440-05/DK/NIDDK NIH HHS; T32CA092347/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/MIRN200 microRNA, human; 0/MicroRNAs; 0/NR5A2 protein, human; 0/PPAR alpha; 0/RNA, Small Interfering; 0/Receptors, Cytoplasmic and Nuclear; 0/nuclear receptor subfamily 0, group B, member 2 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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