Document Detail

Regulation of methotrexate polyglutaminate formation in Ehrlich ascites carcinoma cells by endogenous folate pool.
MedLine Citation:
PMID:  2424199     Owner:  NLM     Status:  MEDLINE    
The conversion of methotrexate to poly-gamma-glutamyl derivatives in Ehrlich ascites carcinoma cells which are characterized by different pools of endogenous folates is described. The cells in which folate pool was high (the 5-fluorodeoxy-uridine-resistant cell line) the ability to convert methotrexate to its polyglutamate derivatives was much lower than in the cells in which folate pool was smaller (the parental cell line). When the cellular folate pool was reduced by treatment of the cells with lysolecithin, a similar methotrexate polyglutamate concentration in both cell lines was observed. These data suggest that cellular folate pool has a regulatory effect on methotrexate polyglutamate synthesis.
M Balińska
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta biochimica Polonica     Volume:  33     ISSN:  0001-527X     ISO Abbreviation:  Acta Biochim. Pol.     Publication Date:  1986  
Date Detail:
Created Date:  1986-06-27     Completed Date:  1986-06-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  14520300R     Medline TA:  Acta Biochim Pol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  31-7     Citation Subset:  IM    
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MeSH Terms
Carcinoma, Ehrlich Tumor / metabolism*
Folic Acid / metabolism*
Methotrexate / analogs & derivatives*,  biosynthesis,  isolation & purification,  metabolism
Peptide Biosynthesis*
Polyglutamic Acid / analogs & derivatives,  biosynthesis*,  isolation & purification
Reg. No./Substance:
25513-46-6/Polyglutamic Acid; 59-05-2/Methotrexate; 59-30-3/Folic Acid; 82334-40-5/methotrexate polyglutamate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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