Document Detail


Regulation of melanocyte apoptosis by Stathmin 1 expression.
MedLine Citation:
PMID:  19017487     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Undesirable hyperpigmentation that can arise from increased melanocyte activity may be alleviated by targeting active melanocytes for apoptosis. The role of Stathmin 1 as an important regulator of microtubule dynamics is well documented. The current study examined the potential of Stathmin 1-targeting strategies in eliminating active melanocytes. A vector to overexpress Stathmin 1 and vectors to express three distinct small hairpin RNAs to knockdown Stathmin 1 expression in normal melanocytes were produced and in cell cultures acted accordingly. Both overexpression and knockdown of Stathmin 1 led to a marked increase in melanocyte apoptosis, as indicated by the accumulation of apoptotic cells and increased levels of cleaved caspase-3. Both up- and down-regulation of Stathmin 1 expression inhibited the activity of differentiated melanocytes, as indicated by decreases in both melanin production and tyrosinase activity. Taken together, these results indicate that hyperactive melanocytes can be inhibited by altering Stathmin 1 expression.
Authors:
Yan Zhang; Jianjun Xiong; Jiali Wang; Xianping Shi; Guodong Bao; Yang Zhang; Zhenyu Zhu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  BMB reports     Volume:  41     ISSN:  1976-6696     ISO Abbreviation:  BMB Rep     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2009-01-16     Revised Date:  2009-04-27    
Medline Journal Info:
Nlm Unique ID:  101465334     Medline TA:  BMB Rep     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  765-70     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Zhongshan Medical College, Sun Yat-sen University, Guangdong, People's Republic of China. zhangyan1981_2003@yahoo.com.cn
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / genetics*
Cell Differentiation / genetics
Cell Proliferation
Cells, Cultured
Gene Expression / physiology
Humans
Melanins / metabolism
Melanocytes / metabolism,  physiology*
Monophenol Monooxygenase / metabolism
Stathmin / genetics,  metabolism,  physiology*
Transfection
Chemical
Reg. No./Substance:
0/Melanins; 0/STMN1 protein, human; 0/Stathmin; EC 1.14.18.1/Monophenol Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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