Document Detail


Regulation of lung surfactant secretion by microRNA-150.
MedLine Citation:
PMID:  22595456     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
P2X7 receptor (P2X7R) is a purinergic ion-channel receptor. We have previously shown that the activation of P2X7R in alveolar type I cells stimulates surfactant secretion in alveolar type II cells. In this study, we determined whether miR-150 regulates P2X7R-mediated surfactant secretion. The miR-150 expression level in alveolar type II cells was much higher than alveolar type I cells, which was inversely correlated with the P2X7R protein level. An adenovirus expressing miR-150 significantly reduced the P2X7R protein expression in E10 cells, an alveolar type I cell line. Furthermore, pre-treatment of E10 cells with the adenovirus reduced the surfactant secretion induced by E10 cell conditioned medium. Our study demonstrates that miR-150 regulates surfactant secretion through P2X7R.
Authors:
Tingting Weng; Amarjit Mishra; Yujie Guo; Yang Wang; Lijing Su; Chaoqun Huang; Chunling Zhao; Xiao Xiao; Lin Liu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-05-14
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  422     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-18     Completed Date:  2012-09-06     Revised Date:  2013-06-24    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  586-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Lundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Culture Media, Conditioned / pharmacology
Humans
Male
MicroRNAs / genetics,  physiology*
Pulmonary Alveoli / secretion*
Pulmonary Surfactant-Associated Proteins / secretion*
Rats
Rats, Sprague-Dawley
Receptors, Purinergic P2X7 / biosynthesis*,  genetics
Respiratory Mucosa / secretion*
Grant Support
ID/Acronym/Agency:
HL-052146/HL/NHLBI NIH HHS; HL-087884/HL/NHLBI NIH HHS; HL-095383/HL/NHLBI NIH HHS; R01 HL052146/HL/NHLBI NIH HHS; R03 HL095383/HL/NHLBI NIH HHS; R21 HL087884/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/MIRN150 microRNA, human; 0/MicroRNAs; 0/Pulmonary Surfactant-Associated Proteins; 0/Receptors, Purinergic P2X7
Comments/Corrections

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