| Regulation of lung surfactant secretion by microRNA-150. | |
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MedLine Citation:
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PMID: 22595456 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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P2X7 receptor (P2X7R) is a purinergic ion-channel receptor. We have previously shown that the activation of P2X7R in alveolar type I cells stimulates surfactant secretion in alveolar type II cells. In this study, we determined whether miR-150 regulates P2X7R-mediated surfactant secretion. The miR-150 expression level in alveolar type II cells was much higher than alveolar type I cells, which was inversely correlated with the P2X7R protein level. An adenovirus expressing miR-150 significantly reduced the P2X7R protein expression in E10 cells, an alveolar type I cell line. Furthermore, pre-treatment of E10 cells with the adenovirus reduced the surfactant secretion induced by E10 cell conditioned medium. Our study demonstrates that miR-150 regulates surfactant secretion through P2X7R. |
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Authors:
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Tingting Weng; Amarjit Mishra; Yujie Guo; Yang Wang; Lijing Su; Chaoqun Huang; Chunling Zhao; Xiao Xiao; Lin Liu |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-05-14 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 422 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-06-18 Completed Date: 2012-09-06 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 586-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Inc. All rights reserved. |
Affiliation:
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Lundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 74078, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Culture Media, Conditioned / pharmacology Humans Male MicroRNAs / genetics, physiology* Pulmonary Alveoli / secretion* Pulmonary Surfactant-Associated Proteins / secretion* Rats Rats, Sprague-Dawley Receptors, Purinergic P2X7 / biosynthesis*, genetics Respiratory Mucosa / secretion* |
| Grant Support | |
ID/Acronym/Agency:
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HL-052146/HL/NHLBI NIH HHS; HL-087884/HL/NHLBI NIH HHS; HL-095383/HL/NHLBI NIH HHS; R01 HL052146/HL/NHLBI NIH HHS; R03 HL095383/HL/NHLBI NIH HHS; R21 HL087884/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/MIRN150 microRNA, human; 0/MicroRNAs; 0/Pulmonary Surfactant-Associated Proteins; 0/Receptors, Purinergic P2X7 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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