Document Detail


Regulation of lung cancer cell migration and invasion by reactive oxygen species and caveolin-1.
MedLine Citation:
PMID:  20923773     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The acquired capability of tumor cells to migrate and invade neighboring tissues is associated with high metastatic potential and advanced stage of cancers. Recently, signaling molecules such as reactive oxygen species (ROS) and caveolin-1 (Cav-1) have been implicated in the aggressive behavior of cancer cells. However, the roles of specific ROS in cancer cell migration and Cav-1 regulation are unclear. We demonstrate here that Cav-1 plays an important role in the migration and invasion of human lung carcinoma H460 cells and that these effects are differentially regulated by cellular ROS. Using various known inhibitors and donors of ROS, we found that different ROS have different effects on Cav-1 expression and cell migration and invasion. Superoxide anion and hydrogen peroxide down-regulated Cav-1 expression and inhibited cell migration and invasion, whereas hydroxyl radical up-regulated the Cav-1 expression and promoted cell migration and invasion. The down-regulating effect of superoxide anion and hydrogen peroxide on Cav-1 is mediated through a transcription-independent mechanism that involves protein degradation via the ubiquitin-proteasome pathway. These results indicate the essential role of different ROS in cancer cell motility and through Cav-1 expression, which may provide a key mechanism controlling tumor progression and metastasis. The up-regulation of Cav-1 and cell motility by hydroxyl free radical suggests an important role of this ROS as a positive regulator of tumor progression.
Authors:
Sudjit Luanpitpong; Siera Jo Talbott; Yon Rojanasakul; Ubonthip Nimmannit; Varisa Pongrakhananon; Liying Wang; Pithi Chanvorachote
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-10-05
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  285     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-06     Completed Date:  2011-01-10     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  38832-40     Citation Subset:  IM    
Affiliation:
Pharmaceutical Technology (International) Program, Chulalongkorn University, Bangkok 10330, Thailand.
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MeSH Terms
Descriptor/Qualifier:
Caveolin 1 / metabolism*
Cell Line, Tumor
Cell Movement
Cell Survival
Down-Regulation
Epithelial Cells / cytology
Gene Expression Regulation, Neoplastic*
Humans
Lung Neoplasms / metabolism,  pathology*
Melanoma / metabolism
Neoplasm Invasiveness
Neoplasm Metastasis
RNA Interference
Reactive Oxygen Species*
Skin Neoplasms / metabolism
Grant Support
ID/Acronym/Agency:
R01-HL076340/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Caveolin 1; 0/Reactive Oxygen Species
Comments/Corrections

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