Document Detail


Regulation of luminal acidification in the male reproductive tract via cell-cell crosstalk.
MedLine Citation:
PMID:  19448084     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the epididymis, spermatozoa acquire their ability to become motile and to fertilize an egg. A luminal acidic pH and a low bicarbonate concentration help keep spermatozoa in a quiescent state during their maturation and storage in this organ. Net proton secretion is crucial to maintain the acidity of the luminal fluid in the epididymis. A sub-population of epithelial cells, the clear cells, express high levels of the proton-pumping V-ATPase in their apical membrane and are important contributors to luminal acidification. This review describes selected aspects of V-ATPase regulation in clear cells. The assembly of a particular set of V-ATPase subunit isoforms governs the targeting of the pump to the apical plasma membrane. Regulation of V-ATPase-dependent proton secretion occurs via recycling mechanisms. The bicarbonate-activated adenylyl cyclase is involved in the non-hormonal regulation of V-ATPase recycling, following activation of bicarbonate secretion by principal cells. The V-ATPase is also regulated in a paracrine manner by luminal angiotensin II by activation of the angiotensin II type 2 receptor (AGTR2), which is located in basal cells. Basal cells have the remarkable property of extending long and slender cytoplasmic projections that cross the tight junction barrier to monitor the luminal environment. Clear cells are activated by a nitric oxide signal that originates from basal cells. Thus, a complex interplay between the different cell types present in the epithelium leads to activation of the luminal acidifying capacity of the epididymis, a process that is crucial for sperm maturation and storage.
Authors:
Winnie W C Shum; Nicolas Da Silva; Dennis Brown; Sylvie Breton
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  212     ISSN:  0022-0949     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-18     Completed Date:  2009-09-02     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1753-61     Citation Subset:  IM    
Affiliation:
Center for Systems Biology, Program in Membrane Biology, Nephrology Division, Massachusetts General Hospital, Boston, MA 02114, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Communication / physiology*
Genitalia, Male / cytology,  physiology*
Hydrogen-Ion Concentration
Male
Mice
Rats
Spermatozoa / physiology
Vacuolar Proton-Translocating ATPases / metabolism
Grant Support
ID/Acronym/Agency:
DK38452/DK/NIDDK NIH HHS; DK43351/DK/NIDDK NIH HHS; DK57521/DK/NIDDK NIH HHS; HD045821/HD/NICHD NIH HHS; HD40793/HD/NICHD NIH HHS; R01 HD040793/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
EC 3.6.1.-/Vacuolar Proton-Translocating ATPases
Comments/Corrections

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