Document Detail


Regulation of liver diphosphofructose phosphatase of the rat and hamster.
MedLine Citation:
PMID:  165727     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The regulatory properties of D-fructose-1, 6-diphosphate 1-phosphohydrolase (FDPase, EC 3.1.3.11) derived from livers of the albino rat and the golden Syrian hamster have been studies. The comparative analysis of the kinetics of saturation by fructose-1, l-diphosphate (FDP) and inhibition by FDP and by the allosteric ligand adenosine 5'-monophosphate (AMP) revealed differences that may have physiological implications. The inhibition studies pointed out that liver phosphatase is under a significantly less stringent regulation in the hibernating rodent. The concentration of AMP required to produce a 50 percent inhibition was 549.5 muM at 37 degrees C, 21.4 muM at 10 degrees C, and 9.2 muM at 5 percent C for the rat; 1,122 muM at 37 degrees C, 34.3 muM at 10 degrees C, and 20.4 muM at 5 degrees C for the hamster. Urea (0.67 M) simulates the effect of low temperature, potentiating the AMP inhibition. At 5 degrees C and 4 M urea, 75 percent loss of activity was found for the rat FDPase in contrast to a 25 percent loss for the hamster. At 37 degrees C, 4 M urea had practically no effect on the FDPase of both species.
Authors:
A Orengo
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The American journal of physiology     Volume:  228     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1975 Apr 
Date Detail:
Created Date:  1975-07-18     Completed Date:  1975-07-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1118-22     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Monophosphate / metabolism
Allosteric Regulation
Animals
Cricetinae / metabolism*
Depression, Chemical
Female
Fructose-Bisphosphatase / metabolism*
Fructosephosphates / metabolism
Kinetics
Liver / enzymology*
Rats / metabolism*
Stimulation, Chemical
Temperature
Urea / pharmacology
Chemical
Reg. No./Substance:
0/Fructosephosphates; 57-13-6/Urea; 61-19-8/Adenosine Monophosphate; EC 3.1.3.11/Fructose-Bisphosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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