Document Detail

Regulation of Krüpple-like factor 5 by targeted protein degradation.
MedLine Citation:
PMID:  20694673     Owner:  NLM     Status:  MEDLINE    
Transcription factors are usually unstable proteins. The degradation of the majority of transcription factors is through the ubiquitin proteasome pathway and is tightly regulated by E3 ubiquitin ligases. KLF5 is an important transcription factor regulating cell proliferation, cell cycle, survival, migration, differentiation, angiogenesis, and stem cell self-renewal. We have shown that the WWP1 E3 ligase targets KLF5 for ubiquitin-mediated degradation. Several methods to determine whether a protein is ubiquitinated have been described [Kaiser, Tagwerker (Methods Enzymol 399:243-248, 2005); Bloom, Pagano (Methods Enzymol 399:249-266, 2005)]. This chapter focuses on experimental approaches testing KLF5 transcription factor ubiquitination and degradation by its E3s.
Ceshi Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  647     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-09     Completed Date:  2010-12-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  267-77     Citation Subset:  IM    
Center for Cell Biology and Cancer Research, Albany Medical College, Albany, NY, USA.
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MeSH Terms
Cell Line, Tumor
Kruppel-Like Transcription Factors / chemistry,  metabolism*
Protein Denaturation
Ubiquitin-Protein Ligases / metabolism
Reg. No./Substance:
0/Kruppel-Like Transcription Factors; EC Ligases

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