| Regulation of leadzyme (lead-dependent ribozyme) reaction by amino acids and rare earth ions. | |
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MedLine Citation:
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PMID: 8841634 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Effects of rare earth ions and amino acids on a novel cleavage reaction of 10mer leadzyme (lead-dependent ribozyme) CUGGGAGUCC have been investigated. Cleavage yield of RNA substrate GGACC decreases GAGCCAG by the leadzyme was 7.4% for 20 min at 25 degrees C. The cleavage reaction was performed at 5 microM leadzyme, 250 nM substrate, 25 microM Pb2+, pH 7.5. Under the same reaction condition, the addition of 25 microM Nd3+ led to the increase of the yield from 7.4% to 39.8 %, although other divalent metal ions such as Mn2+ and Co2+ showed no effect on the yield. At 75 microM Pb2+, the yield decreased from 48.0 % to 15.0 % by the addition of 10 mM Arg, although the addition of 10 mM Lys did not affect the yield. These results indicate that the cleavage reaction by the leadzyme is regulated by the combination of metal ions and amino acids. |
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Authors:
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T Ohmichi; M Sasaki; N Sugimoto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nucleic acids symposium series Volume: - ISSN: 0261-3166 ISO Abbreviation: Nucleic Acids Symp. Ser. Publication Date: 1995 |
Date Detail:
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Created Date: 1996-12-18 Completed Date: 1996-12-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8007206 Medline TA: Nucleic Acids Symp Ser Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 227-8 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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pharmacology Animals Base Sequence Binding Sites Kinetics Lead / metabolism*, pharmacology Magnesium / metabolism Metals, Rare Earth / pharmacology Nucleic Acid Conformation RNA, Catalytic / chemistry, genetics, metabolism* Substrate Specificity Tetrahymena / enzymology |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Metals, Rare Earth; 0/RNA, Catalytic; 7439-92-1/Lead; 7439-95-4/Magnesium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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