Document Detail

Regulation of leadzyme (lead-dependent ribozyme) reaction by amino acids and rare earth ions.
MedLine Citation:
PMID:  8841634     Owner:  NLM     Status:  MEDLINE    
Effects of rare earth ions and amino acids on a novel cleavage reaction of 10mer leadzyme (lead-dependent ribozyme) CUGGGAGUCC have been investigated. Cleavage yield of RNA substrate GGACC decreases GAGCCAG by the leadzyme was 7.4% for 20 min at 25 degrees C. The cleavage reaction was performed at 5 microM leadzyme, 250 nM substrate, 25 microM Pb2+, pH 7.5. Under the same reaction condition, the addition of 25 microM Nd3+ led to the increase of the yield from 7.4% to 39.8 %, although other divalent metal ions such as Mn2+ and Co2+ showed no effect on the yield. At 75 microM Pb2+, the yield decreased from 48.0 % to 15.0 % by the addition of 10 mM Arg, although the addition of 10 mM Lys did not affect the yield. These results indicate that the cleavage reaction by the leadzyme is regulated by the combination of metal ions and amino acids.
T Ohmichi; M Sasaki; N Sugimoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleic acids symposium series     Volume:  -     ISSN:  0261-3166     ISO Abbreviation:  Nucleic Acids Symp. Ser.     Publication Date:  1995  
Date Detail:
Created Date:  1996-12-18     Completed Date:  1996-12-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8007206     Medline TA:  Nucleic Acids Symp Ser     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  227-8     Citation Subset:  IM    
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
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MeSH Terms
Amino Acids / pharmacology
Base Sequence
Binding Sites
Lead / metabolism*,  pharmacology
Magnesium / metabolism
Metals, Rare Earth / pharmacology
Nucleic Acid Conformation
RNA, Catalytic / chemistry,  genetics,  metabolism*
Substrate Specificity
Tetrahymena / enzymology
Reg. No./Substance:
0/Amino Acids; 0/Metals, Rare Earth; 0/RNA, Catalytic; 7439-92-1/Lead; 7439-95-4/Magnesium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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