Document Detail

Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil.
MedLine Citation:
PMID:  22556214     Owner:  NLM     Status:  MEDLINE    
Omega-3-PUFAs, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), are associated with prevention of various aspects of metabolic syndrome. In the present studies, the effects of oil rich in EPA on gene expression and activation of nuclear receptors was examined and compared with other ω3-PUFAs. The EPA-rich oil (EO) altered the expression of FA metabolism genes in THP-1 cells, including stearoyl CoA desaturase (SCD) and FA desaturase-1 and -2 (FASDS1 and -2). Other ω3-PUFAs resulted in a similar gene expression response for a subset of genes involved in lipid metabolism and inflammation. In reporter assays, EO activated human peroxisome proliferator-activated receptor α (PPARα) and PPARβ/γ with minimal effects on PPARγ, liver X receptor, retinoid X receptor, farnesoid X receptor, and retinoid acid receptor γ (RARγ); these effects were similar to that observed for purified EPA. When serum from a 6 week clinical intervention with dietary supplements containing olive oil (control), DHA, or two levels of EPA were applied to THP-1 cells, the expression of SCD and FADS2 decreased in the cells treated with serum from the ω3-PUFA-supplemented individuals. Taken together, these studies indicate regulation of gene expression by EO that is consistent with treating aspects of dyslipidemia and inflammation.
Peter J Gillies; Sujata K Bhatia; Leigh A Belcher; Daniel B Hannon; Jerry T Thompson; John P Vanden Heuvel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-03
Journal Detail:
Title:  Journal of lipid research     Volume:  53     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-16     Completed Date:  2012-11-23     Revised Date:  2013-08-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1679-89     Citation Subset:  IM    
Central Research and Development, DuPont Experimental Station, E328/140B, Wilmington, DE 19880, USA.
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MeSH Terms
Cell Line
Dietary Supplements / analysis
Eicosapentaenoic Acid / analysis*,  blood,  chemistry,  pharmacology
Inflammation / genetics,  metabolism
Lipid Metabolism / drug effects*,  genetics*
Oils / chemistry*,  pharmacology*
Structure-Activity Relationship
Transcription Factors / metabolism
Transcriptome / drug effects
Reg. No./Substance:
0/Oils; 0/Transcription Factors; 1553-41-9/Eicosapentaenoic Acid

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