Document Detail

Regulation of CXCL9/10/11 in oral keratinocytes and fibroblasts.
MedLine Citation:
PMID:  19029086     Owner:  NLM     Status:  MEDLINE    
Th1 and Th2 cytokines such as interferon-gamma (IFN-gamma ) , tumor necrosis factor- alpha (TNF-alpha ), and IL-4 are expressed in T-cell-mediated inflammation in the oral cavity. We tested the hypothesis that those cytokines may act on CXCR3-agonistic chemokines, T-cell recruiting factors, and on neighboring cells, including oral keratinocytes and fibroblasts. Human immortalized oral keratinocytes (RT7) and fibroblasts (GT1) after 24-hour stimulation with IFN-gamma showed increased mRNA levels of CXCL9 (600- and 700-fold), CXCL10 (10,000- and 150-fold), and CXCL11 (5000- and 300-fold), respectively. In contrast, TNF-alpha caused an increase in CXCL9 (300-fold), CXCL10 (2000-fold), and CXCL11 (2000-fold) mRNA levels in GT1, but not RT7 cells, at 24 hrs. IL-4 reinforced the promotion of CXCL9, CXCL10, and CXCL11 expression by IFN-gamma in RT7 cells, whereas IL-4 inhibited the increased levels by IFN-gamma and TNF-alpha in GT1 cells. Thus, IFN-gamma , TNF-alpha , and IL-4 appear cooperatively to regulate CXCR3-agonistic chemokines in oral keratinocytes and fibroblasts in T-cell-mediated oral inflammation sites.
K Ohta; H Shigeishi; M Taki; H Nishi; K Higashikawa; M Takechi; N Kamata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dental research     Volume:  87     ISSN:  1544-0591     ISO Abbreviation:  J. Dent. Res.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-25     Completed Date:  2009-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0354343     Medline TA:  J Dent Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1160-5     Citation Subset:  D; IM    
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Minami-Ku, Hiroshima 734-8553, Japan.
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MeSH Terms
Cell Line
Chemokine CXCL10 / immunology*
Chemokine CXCL11 / immunology*
Chemokine CXCL9 / immunology*
Fibroblasts / immunology*
Gingiva / immunology*,  pathology
Interferon-gamma / immunology
Interleukin-4 / immunology
Keratinocytes / immunology*
Lymphocyte Activation / immunology
Mouth Mucosa / immunology*,  pathology
Receptors, CXCR3 / agonists
Th1 Cells / immunology
Th2 Cells / immunology
Time Factors
Tumor Necrosis Factor-alpha / immunology
Reg. No./Substance:
0/CXCL10 protein, human; 0/CXCL11 protein, human; 0/CXCL9 protein, human; 0/CXCR3 protein, human; 0/Chemokine CXCL10; 0/Chemokine CXCL11; 0/Chemokine CXCL9; 0/Receptors, CXCR3; 0/Tumor Necrosis Factor-alpha; 207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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