Document Detail


Regulation of hexose transport in cultured bovine retinal microvessel endothelium by insulin.
MedLine Citation:
PMID:  3539629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In microvessel endothelium obtained from young calf retinas, insulin stimulated the uptake of 2-deoxy-D-glucose in a dose-dependent manner. The maximal level of glucose uptake (50-100% over basal values) was observed at 1 ng ml-1 insulin. The effects of insulin on glucose uptake were time-dependent, with optimum stimulation of glucose transport observed after 90 min of insulin treatment. The effects of insulin were prevented by 15 min pretreatment with 2 micrograms ml-1 cycloheximide or by 2 micrograms ml-1 actinomycin D, suggesting a role for new protein synthesis in the actions of insulin on glucose transport in this cell type. These results demonstrate that cultured retinal microvessel endothelial cells exhibit an insulin-sensitive glucose transport system.
Authors:
L A Allen; M E Gerritsen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental eye research     Volume:  43     ISSN:  0014-4835     ISO Abbreviation:  Exp. Eye Res.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1987-02-11     Completed Date:  1987-02-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370707     Medline TA:  Exp Eye Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  679-86     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
3-O-Methylglucose
Animals
Cattle
Cells, Cultured
Cycloheximide / pharmacology
Dactinomycin / pharmacology
Deoxyglucose / metabolism
Dose-Response Relationship, Drug
Endothelium / drug effects,  metabolism
Glucose / metabolism*
Insulin / pharmacology*
Methylglucosides / metabolism
Retinal Vessels / drug effects,  metabolism*
Stimulation, Chemical
Time Factors
Chemical
Reg. No./Substance:
0/Methylglucosides; 11061-68-0/Insulin; 146-72-5/3-O-Methylglucose; 154-17-6/Deoxyglucose; 50-76-0/Dactinomycin; 50-99-7/Glucose; 66-81-9/Cycloheximide; 7658-08-4/6-deoxyglucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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