Document Detail

Regulation of herpesvirus macromolecular synthesis. VI. Synthesis and modification of viral polypeptides in enucleated cells.
MedLine Citation:
PMID:  195087     Owner:  NLM     Status:  MEDLINE    
Cells were enucleated with cytochalasin B after infection with herpes simplex virus 1. When protein synthesis was blocked by cycloheximide from the time of infection, mRNA for viral alpha-infected cell polypeptides (ICP) 4, 0, and 27 accumulated in the cytoplasm and was expressed after the removal of both drug and nucleus. A host protein, ICP 22, whose synthesis is stimulated in intact cells, was not made, and viral protein ICP4, which is normally modified to a form that migrates more slowly in polyacrylamide gels, was not modified in the absence of the nucleus. After enucleation at 2 h postinfection, a number of viral beta and gamma proteins continued to be made, starting at 20 to 25% of the normal rates and declining with a half-time of about 2 h. The synthesis of ICP 4 declined more rapidly, suggesting that it is switched off in the cytoplasm.
M Fenwick; B Roizman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  22     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1977 Jun 
Date Detail:
Created Date:  1977-08-25     Completed Date:  1977-08-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  720-5     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Nucleus / drug effects
Cycloheximide / pharmacology
Cytochalasin B / pharmacology
Cytoplasm / metabolism
Peptide Biosynthesis*
RNA, Messenger / biosynthesis
RNA, Viral / biosynthesis
Simplexvirus / metabolism*
Viral Proteins / biosynthesis*
Reg. No./Substance:
0/RNA, Messenger; 0/RNA, Viral; 0/Viral Proteins; 14930-96-2/Cytochalasin B; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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