Document Detail

Regulation of hepatic mRNA levels for the growth hormone receptor in rats with altered thyroid status.
MedLine Citation:
PMID:  8745145     Owner:  NLM     Status:  MEDLINE    
Changes in thyroid status have a major effect on the GH/IGF-I axis. In the rat, there is a single gene for the growth hormone receptor (GHR), that is transcribed into two different sized mRNA transcripts following alternative splicing, one transcript codes for the GHR (4.0-4.5 kb) and the other growth hormone binding protein (GHBP) (1.2-1.3 kb). We have studied the regulation of hepatic GHR gene expression by thyroid hormones in male Wistar rats rendered hypothyroid (n = 6) and hyperthyroid (n = 6) compared to controls (n = 6). By northern blot analysis, two transcripts with an estimated size of 4.2 and 1.2 kb, respectively, were detected in all groups. Hyperthyroidism was associated with a significant increase in the 4.2 kb transcript compared to hypothyroidism (P < 0.05), but no changes were observed in the 1.2 kb transcript. Thus, our results suggest that in the rat hyperthyroidism is associated with either increased hepatic gene transcription or decreased clearance of the 4.2 kb transcript for the GHR compared with hypothyroidism.
X Y Shen; J Rodriguez-Arnao; R J Ross
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Growth regulation     Volume:  5     ISSN:  0956-523X     ISO Abbreviation:  Growth Regul.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-10-11     Completed Date:  1996-10-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9106990     Medline TA:  Growth Regul     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  199-202     Citation Subset:  IM    
University Department of Medicine, Clinical Sciences Centre, Northern General Hospital, Sheffield, UK.
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MeSH Terms
Alternative Splicing
Analysis of Variance
Gene Expression Regulation* / drug effects
Hyperthyroidism / chemically induced,  metabolism*
Hypothyroidism / chemically induced,  metabolism*
Liver / drug effects,  metabolism*
Propylthiouracil / pharmacology
RNA, Messenger / metabolism*
Rats, Wistar
Receptors, Somatotropin / biosynthesis*
Reference Values
Thyroid Gland / physiology*,  physiopathology
Thyroxine / pharmacology
Transcription, Genetic*
Reg. No./Substance:
0/RNA, Messenger; 0/Receptors, Somatotropin; 51-52-5/Propylthiouracil; 7488-70-2/Thyroxine

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