Document Detail


Regulation of hemopexin transcription by calcium ionophores and phorbol ester in hepatoma cells.
MedLine Citation:
PMID:  12850286     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hemopexin (Hx) is an acute-phase hepatic protein, whose transcription is upregulated by IL-6. The transcription rate of Hx was found to be increased 11-fold by the calcium ionophore A23187, 25-fold by the calcium ionophore ionomycin, and 4-5-fold by phorbol 12-myristate 13-acetate (PMA) in serum-starved H4IIE rat hepatoma cells. Insulin did not affect the transcription rate of Hx. These findings are consistent with involvement of intracellular calcium concentrations and activation of protein kinase C (PKC) action in the regulation of Hx.
Authors:
Susan E Stred; Deborah Cote; Ruth S Weinstock; Joseph L Messina
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  204     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-07-09     Completed Date:  2004-04-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  111-6     Citation Subset:  IM    
Affiliation:
Cell and Molecular Biology Program and Department of Pediatrics, SUNY Upstate Medical University, Syracuse, NY 13210, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism
Carcinoma, Hepatocellular / pathology*
Cell Line, Tumor
Gene Expression Regulation* / drug effects
Hemopexin / genetics*
Insulin / pharmacology
Ionophores / pharmacology
Kinetics
Protein Kinase C / metabolism
Rats
Tetradecanoylphorbol Acetate / pharmacology
Transcription, Genetic* / drug effects
Grant Support
ID/Acronym/Agency:
DK02114/DK/NIDDK NIH HHS; DK54440/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Ionophores; 11061-68-0/Insulin; 16561-29-8/Tetradecanoylphorbol Acetate; 7440-70-2/Calcium; 9013-71-2/Hemopexin; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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