Document Detail


Regulation of growth factors in hormone- and drug-resistant prostate cancer cells by synergistic combination of docetaxel and octreotide.
MedLine Citation:
PMID:  19191785     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE To evaluate the effects of combined treatment with docetaxel and octreotide, a somatostatin analogue, on human hormone- and drug-refractory prostate cancer cell lines, PC-3 and DU-145, and on some growth factors related to tumour growth and angiogenesis in prostate cancer. MATERIALS AND METHODS A cell proliferation assay was used to assess the cytotoxicity of the drugs. To verify apoptosis, both DNA fragmentation (by enzyme-linked immunosorbent assay) and caspase 3/7 activity were measured. We also investigated the effect of combined docetaxel and octreotide on growth factors secreted from prostate cancer cells using a human growth factor antibody array. RESULTS The combination of docetaxel and octreotide resulted in significant synergistic cytotoxic activity and apoptosis, which was dose- and time-dependent. The combined treatment also resulted in significantly less secretion of stem cell factor and platelet-derived growth factor-AB in PC-3 cells, and transforming growth factor-beta and basic fibroblast growth factor in DU-145 cells, than in untreated controls. CONCLUSION Octreotide, a somatostatin analogue, combined with docetaxel might provide a rationale treatment option for hormone-refractory prostate cancer cells, not only by direct inhibition of cell proliferation but also by inhibiting the secretion of growth factors.
Authors:
Cigdem Erten; Burcak Karaca; Yuksel Kucukzeybek; Gurbuz Gorumlu; Ercument Cengiz; Mustafa K Gul; Harika Atmaca; Selim Uzunoglu; Bulent Karabulut; Ulus A Sanli; Ruchan Uslu
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Publication Detail:
Type:  Journal Article     Date:  2009-02-03
Journal Detail:
Title:  BJU international     Volume:  104     ISSN:  1464-410X     ISO Abbreviation:  BJU Int.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-08-03     Completed Date:  2009-09-02     Revised Date:  2013-05-28    
Medline Journal Info:
Nlm Unique ID:  100886721     Medline TA:  BJU Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  107-14     Citation Subset:  IM    
Affiliation:
Division of Medical Oncology, Tulay Aktas Oncology Hospital, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Combined Chemotherapy Protocols / therapeutic use*
Cell Line, Tumor
Drug Resistance, Neoplasm / drug effects*
Drug Synergism
Enzyme-Linked Immunosorbent Assay
Humans
Intercellular Signaling Peptides and Proteins / metabolism*
Male
Neoplasms, Hormone-Dependent / drug therapy*,  metabolism,  pathology
Octreotide / administration & dosage
Prostate / metabolism*,  pathology
Prostatic Neoplasms / drug therapy*,  metabolism,  pathology
Taxoids / administration & dosage
Treatment Outcome
Chemical
Reg. No./Substance:
0/Intercellular Signaling Peptides and Proteins; 0/Taxoids; 15H5577CQD/docetaxel; 83150-76-9/Octreotide

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