| Regulation of growth factors in hormone- and drug-resistant prostate cancer cells by synergistic combination of docetaxel and octreotide. | |
| | |
MedLine Citation:
|
PMID: 19191785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE To evaluate the effects of combined treatment with docetaxel and octreotide, a somatostatin analogue, on human hormone- and drug-refractory prostate cancer cell lines, PC-3 and DU-145, and on some growth factors related to tumour growth and angiogenesis in prostate cancer. MATERIALS AND METHODS A cell proliferation assay was used to assess the cytotoxicity of the drugs. To verify apoptosis, both DNA fragmentation (by enzyme-linked immunosorbent assay) and caspase 3/7 activity were measured. We also investigated the effect of combined docetaxel and octreotide on growth factors secreted from prostate cancer cells using a human growth factor antibody array. RESULTS The combination of docetaxel and octreotide resulted in significant synergistic cytotoxic activity and apoptosis, which was dose- and time-dependent. The combined treatment also resulted in significantly less secretion of stem cell factor and platelet-derived growth factor-AB in PC-3 cells, and transforming growth factor-beta and basic fibroblast growth factor in DU-145 cells, than in untreated controls. CONCLUSION Octreotide, a somatostatin analogue, combined with docetaxel might provide a rationale treatment option for hormone-refractory prostate cancer cells, not only by direct inhibition of cell proliferation but also by inhibiting the secretion of growth factors. |
| | |
Authors:
|
Cigdem Erten; Burcak Karaca; Yuksel Kucukzeybek; Gurbuz Gorumlu; Ercument Cengiz; Mustafa K Gul; Harika Atmaca; Selim Uzunoglu; Bulent Karabulut; Ulus A Sanli; Ruchan Uslu |
Related Documents
:
|
2045475 - Estradiol and progesterone receptors in cultured normal human breast epithelial cells a... 9168005 - Neoplastic transformation of cultured mammalian cells by estrogens and estrogenlike che... 14656475 - Mullerian inhibiting substance induces nfkb signaling in breast and prostate cancer cells. 17121935 - Absorption and subcellular localization of lycopene in human prostate cancer cells. 12972555 - Spy1 interacts with p27kip1 to allow g1/s progression. 2045475 - Estradiol and progesterone receptors in cultured normal human breast epithelial cells a... |
Publication Detail:
|
Type: Journal Article Date: 2009-02-03 |
Journal Detail:
|
Title: BJU international Volume: 104 ISSN: 1464-410X ISO Abbreviation: BJU Int. Publication Date: 2009 Jul |
Date Detail:
|
Created Date: 2009-08-03 Completed Date: 2009-09-02 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100886721 Medline TA: BJU Int Country: England |
Other Details:
|
Languages: eng Pagination: 107-14 Citation Subset: IM |
Affiliation:
|
Division of Medical Oncology, Tulay Aktas Oncology Hospital, Turkey. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antineoplastic Combined Chemotherapy Protocols
/
therapeutic use* Cell Line, Tumor Drug Resistance, Neoplasm / drug effects* Drug Synergism Enzyme-Linked Immunosorbent Assay Humans Intercellular Signaling Peptides and Proteins / metabolism* Male Neoplasms, Hormone-Dependent / drug therapy*, metabolism, pathology Octreotide / administration & dosage Prostate / metabolism*, pathology Prostatic Neoplasms / drug therapy*, metabolism, pathology Taxoids / administration & dosage Treatment Outcome |
| Chemical | |
Reg. No./Substance:
|
0/Intercellular Signaling Peptides and Proteins; 0/Taxoids; 114977-28-5/docetaxel; 83150-76-9/Octreotide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Can contemporary transrectal prostate biopsy accurately select candidates for hemi-ablative focal th...
Next Document: Listeria monocytogenes infection in the face of innate immunity.