Document Detail


Regulation of growth factors associated cell migration by C-phycocyanin scaffold in dermal wound healing.
MedLine Citation:
PMID:  21995435     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
1. The present study examined the role of C-phycocyanin (C-pc) on growth factors and cell migration during wound healing. 2. Histological and biochemical studies revealed that C-pc scaffold significantly (P<0.01) increased hydroxyl proline, total hexamine and protein content and decreased uronic acid content in the wound tissues during time course study in newly formed skin. 3. RT(2) PCR array of mouse growth factors in wound tissue demonstrated over expression (up to 10 fold) of growth factors such as Cxcl12, Fgf18, Lefty 1, Lefty 2, Rabep 1 and Zip91 and down regulation (up to -10 fold) of Amh, Bmp 7 and Nodal genes in 6 days period, in C-pc treated groups. Besides, Csf 3, Fgf 22, Mdk, Igf 2, Tgfα 1 and Il1 β showed an up-regulation of more than 30 fold, than the control groups. Tgf β subfamily cytokine growth factors such as Bmp 2, 4 and 8b and other growth factors like Cxcl 1 showed highest activity in day 3 indicating transient type of regulation. Western blot analysis showed positive correlation between gene activity and protein expressions of Bmp 8b, Bmp4, Bmp2, Cxcl 1. Day 6 in C-pc group revealed highest csf3 and IL1β expression. 4. C-pc had no direct effect on keratinocyte migration. However, keratinocytes that were co-cultured with fibroblasts demonstrated significantly higher rate of migration in the presence of C-pc, indicating an indirect effect of C-pc on keratinocyte migration. 5. In conclusion, biodegradable C-pc scaffold may help to serve as an alternate scaffold material for wound healing.
Authors:
H Madhyastha; R Madhyastha; Y Nakajima; S Omura; M Maruyama
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-13
Journal Detail:
Title:  Clinical and experimental pharmacology & physiology     Volume:  -     ISSN:  1440-1681     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0425076     Medline TA:  Clin Exp Pharmacol Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Clinical and Experimental Pharmacology and Physiology © 2011 Blackwell Publishing Asia Pty Ltd.
Affiliation:
Department of Applied Physiology Faculty of Medicine University of Miyazaki Miyazaki Japan.
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