Document Detail


Regulation of granulosa and theca cell transcriptomes during ovarian antral follicle development.
MedLine Citation:
PMID:  18288646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Coordinated interactions between ovarian granulosa and theca cells are required for female endocrine function and fertility. To elucidate these interactions the regulation of the granulosa and theca cell transcriptomes during bovine antral follicle development were investigated. Granulosa cells and theca cells were isolated from small (<5 mm), medium (5-10 mm), and large (>10 mm) antral bovine follicles. A microarray analysis of 24,000 bovine genes revealed that granulosa cells and theca cells each had gene sets specific to small, medium and large follicle cells. Transcripts regulated (i.e., minimally changed 1.5-fold) during antral follicle development for the granulosa cells involved 446 genes and for theca cells 248 genes. Only 28 regulated genes were common to both granulosa and theca cells. Regulated genes were functionally categorized with a focus on growth factors and cytokines expressed and regulated by the two cell types. Candidate regulatory growth factor proteins mediating both paracrine and autocrine cell-cell interactions include macrophage inflammatory protein (MIP1 beta), teratocarcinoma-derived growth factor 1 (TDGF1), stromal derived growth factor 1 (SDF1; i.e., CXCL12), growth differentiation factor 8 (GDF8), glia maturation factor gamma (GMFG), osteopontin (SPP1), angiopoietin 4 (ANGPT4), and chemokine ligands (CCL 2, 3, 5, and 8). The current study examined granulosa cell and theca cell regulated genes associated with bovine antral follicle development and identified candidate growth factors potentially involved in the regulation of cell-cell interactions required for ovarian function.
Authors:
Michael K Skinner; Michelle Schmidt; Marina I Savenkova; Ingrid Sadler-Riggleman; Eric E Nilsson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  75     ISSN:  1098-2795     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-06-23     Completed Date:  2008-09-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1457-72     Citation Subset:  IM    
Affiliation:
Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4231, USA. skinner@mail.wsu.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Autocrine Communication / genetics
Cattle
Cluster Analysis
Female
Gene Expression Profiling*
Gene Expression Regulation*
Granulosa Cells / metabolism*
Models, Biological
Oligonucleotide Array Sequence Analysis
Ovarian Follicle / growth & development*,  metabolism
Paracrine Communication / genetics
Signal Transduction / genetics
Theca Cells / metabolism*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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