| Regulation of gonadotropin-releasing hormone-1 gene transcription by members of the purine-rich element-binding protein family. | |
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MedLine Citation:
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PMID: 19996387 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Gonadotropin-releasing hormone-1 (GnRH1) controls reproduction by stimulating the release of gonadotropins from the pituitary. To characterize regulatory factors governing GnRH1 gene expression, we employed biochemical and bioinformatics techniques to identify novel GnRH1 promoter-binding proteins from the brain of the cichlid fish, Astatotilapia burtoni (A. burtoni). Using an in vitro DNA-binding assay followed by mass spectrometric peptide mapping, we identified two members of the purine-rich element-binding (Pur) protein family, Puralpha and Purbeta, as candidates for GnRH1 promoter binding and regulation. We found that transcripts for both Puralpha and Purbeta colocalize in GnRH1-expressing neurons in the preoptic area of the hypothalamus in A. burtoni brain. Furthermore, we confirmed in vivo binding of endogenous Puralpha and Purbeta to the upstream region of the GnRH1 gene in A. burtoni brain and mouse neuronal GT1-7 cells. Consistent with the relative promoter occupancy exhibited by endogenous Pur proteins, overexpression of Purbeta, but not Puralpha, significantly downregulated GnRH1 mRNA levels in transiently transfected GT1-7 cells, suggesting that Purbeta acts as a repressor of GnRH1 gene transcription. |
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Authors:
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Sheng Zhao; Robert J Kelm; Russell D Fernald |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-12-08 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 298 ISSN: 1522-1555 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-22 Completed Date: 2010-03-30 Revised Date: 2011-07-25 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E524-33 Citation Subset: IM |
Affiliation:
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Dept. of Biology, Stanford University, California, 94305-5020, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / physiology* Cichlids / physiology* DNA-Binding Proteins / metabolism* Gene Expression Regulation / physiology* Gonadotropin-Releasing Hormone / metabolism* Mice Nerve Tissue Proteins / metabolism Protein Precursors / metabolism* Transcriptional Activation / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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HL-054281/HL/NHLBI NIH HHS; NS 34950/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Nerve Tissue Proteins; 0/Protein Precursors; 0/Pura protein, mouse; 0/Purb protein, mouse; 0/progonadoliberin I; 33515-09-2/Gonadotropin-Releasing Hormone |
| Comments/Corrections | |
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