Document Detail


Regulation of glypican-1, syndecan-1 and syndecan-4 mRNAs expression by follicle-stimulating hormone, cAMP increase and calcium influx during rat Sertoli cell development.
MedLine Citation:
PMID:  12135485     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In seminiferous tubules, Sertoli cells provide structural and nutritional support for the developing germinal cells. Cell- to-cell signaling and cell adhesion require proteoglycans expressed at the cell membrane. A preliminary biochemical and structural approach indicated that cell surface proteoglycans are mostly heparan sulfate proteoglycans (HSPG). Glypican-1, syndecans-1 and -4 were identified using a molecular approach. Their differential regulation was demonstrated in immature rat Sertoli cells. Follicle-stimulating hormone (FSH) is the main regulator of Sertoli cell function. Signal transduction triggered by FSH involves both an increased intracellular cAMP synthesis and a calcium influx. This study demonstrates that FSH, through its second messengers (increase in intracellular cAMP and intracellular calcium), downregulated the glypican-1 mRNA expression in Sertoli cells from 20-day-old rats. On the other hand, syndecan-1 mRNA expression is not modulated by FSH as it would result from the antagonistic effects of increased intracellular cAMP and intracellular calcium levels. Finally, syndecan-4 mRNA expression is not regulated by this pathway. The present study was extended during Sertoli cell development. Indeed, Sertoli cells undergo extensive changes during the postnatal period both in structure and function. These important transformations are critical for the establishment of spermatogenesis and development of the adult pattern of testicular function. Our data indicated that the regulation of HSPG mRNA expression is HSPG-specific and depends on the Sertoli cell developmental stage.
Authors:
Sylvie Brucato; Jean Bocquet; Corinne Villers
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  269     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-07-23     Completed Date:  2002-09-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3461-9     Citation Subset:  IM    
Affiliation:
Laboratoire de Biochimie IRBA, UPRES, Université de Caen, France. s_brucato@yahoo.fr
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MeSH Terms
Descriptor/Qualifier:
3',5'-Cyclic-AMP Phosphodiesterases / antagonists & inhibitors
4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone / pharmacology
Animals
Bucladesine / pharmacology
Calcium Signaling / drug effects*
Cholera Toxin / pharmacology
Cyclic AMP / physiology*
Enzyme Inhibitors / pharmacology
Follicle Stimulating Hormone / pharmacology*
Gene Expression Regulation / drug effects*
Heparan Sulfate Proteoglycans / biosynthesis,  genetics*
Male
Membrane Glycoproteins / biosynthesis,  genetics*
Proteoglycans / biosynthesis,  genetics*
RNA, Messenger / biosynthesis*,  genetics
Rats
Rats, Sprague-Dawley
Second Messenger Systems / physiology*
Sertoli Cells / drug effects*,  metabolism
Syndecan-1
Syndecan-4
Syndecans
Testis / cytology,  growth & development
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Heparan Sulfate Proteoglycans; 0/Membrane Glycoproteins; 0/Proteoglycans; 0/RNA, Messenger; 0/Sdc1 protein, rat; 0/Sdc4 protein, rat; 0/Syndecan-1; 0/Syndecan-4; 0/Syndecans; 29925-17-5/4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone; 362-74-3/Bucladesine; 60-92-4/Cyclic AMP; 9002-68-0/Follicle Stimulating Hormone; 9012-63-9/Cholera Toxin; EC 3.1.4.17/3',5'-Cyclic-AMP Phosphodiesterases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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