Document Detail


Regulation of FAT/CD36 gene expression: further evidence in support of a role of the protein in fatty acid binding/transport.
MedLine Citation:
PMID:  9250603     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Much biochemical evidence has implicated rat adipocyte CD36 (FAT) in membrane binding and transport of long-chain fatty acids (FA). Expression of the mRNA favored tissues with active FA metabolism and was upregulated in vivo with diabetes and with high fat feeding. In culture, CD36 mRNA was a strong marker of preadipocyte differentiation and was modulated by the same factors effective on mRNAs coding for other proteins involved in FA metabolism. In preadipocytes, long-chain FA or 2-bromopalmitate but not short-chain FA strongly induced CD36 mRNA within 8 h to an optimum within 24 h. Removal of the FA resulted in a decay of CD36 mRNA with a half life of about 12 h. In differentiated adipocytes, levels of CD36 mRNA were downregulated by the 3': 5'-cyclic adenosine monophosphate, cAMP, analog, 8-(4-chlorophenylthio) adenosine, 8-CPT, at concentrations of 1-100 microM. The effect, observed within 6 h, was optimal after 18 h and independent of the action of 8-CPT to mobilize FA. Regulation of CD36 expression by factors effective on expression of other proteins implicated in FA metabolism is consistent with its role in membrane FA transport.
Authors:
Z Sfeir; A Ibrahimi; E Amri; P Grimaldi; N Abumrad
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Prostaglandins, leukotrienes, and essential fatty acids     Volume:  57     ISSN:  0952-3278     ISO Abbreviation:  Prostaglandins Leukot. Essent. Fatty Acids     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-10-01     Completed Date:  1997-10-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8802730     Medline TA:  Prostaglandins Leukot Essent Fatty Acids     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  17-21     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, State University of New York at Stony Brook, 11733, USA.
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / metabolism*
Animals
Antigens, CD36 / genetics*
Biological Transport
Cell Line
Cyclic AMP / pharmacology
Fatty Acids / metabolism*
Gene Expression Regulation* / drug effects
Kinetics
Membrane Glycoproteins / genetics*
Organic Anion Transporters*
RNA, Messenger / biosynthesis
Rats
Stem Cells / metabolism
Grant Support
ID/Acronym/Agency:
DK33301/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD36; 0/Fatty Acids; 0/Membrane Glycoproteins; 0/Organic Anion Transporters; 0/RNA, Messenger; 60-92-4/Cyclic AMP

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