| Regulation of the functional interaction between cyclin D1 and the estrogen receptor. | |
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MedLine Citation:
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PMID: 11073968 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report that the functional interaction between cyclin D1 and the estrogen receptor (ER) is regulated by a signal transduction pathway involving the second messenger, cyclic AMP (cAMP). The cell-permeable cAMP analogue 8-bromo-cAMP caused a concentration-dependent enhancement of cyclin D1-ER complex formation, as judged both by coimmunoprecipitation and mammalian two-hybrid analysis. This effect was paralleled by increases in ligand-independent ER-mediated transcription from an estrogen response element containing reporter construct. These effects of 8-bromo-cAMP were antagonized by a specific protein kinase A (PKA) inhibitor, indicating that the signaling pathway involved was PKA dependent. Further, we show that culture of MCF-7 cells on a cellular substratum of murine preadipocytes also enhanced the functional interaction between cyclin D1 and ER in a PKA-dependent manner. These findings demonstrate a collaboration between cAMP signaling and cyclin D1 in the ligand-independent activation of ER-mediated transcription in mammary epithelial cells and show that the functional associations of cyclin D1 are regulated as a function of cellular context. |
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Authors:
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J Lamb; M H Ladha; C McMahon; R L Sutherland; M E Ewen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biology Volume: 20 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2000-12-19 Completed Date: 2000-12-19 Revised Date: 2013-04-17 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 8667-75 Citation Subset: IM |
Affiliation:
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Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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8-Bromo Cyclic Adenosine Monophosphate
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pharmacology Adipocytes / metabolism Animals Breast / metabolism Breast Neoplasms / metabolism* Cell Communication Cells, Cultured Coculture Techniques Cyclin D1 / metabolism* Epithelial Cells / metabolism Estradiol / pharmacology Humans Ligands Mice Protein Binding / drug effects Rats Receptors, Estrogen / metabolism* Stromal Cells / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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P01 CA80111/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ligands; 0/Receptors, Estrogen; 136601-57-5/Cyclin D1; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 50-28-2/Estradiol |
| Comments/Corrections | |
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