Document Detail

Regulation of fibroblast growth factor 2 expression in melanoma cells by the c-MYB proto-oncoprotein.
MedLine Citation:
PMID:  9372243     Owner:  NLM     Status:  MEDLINE    
Dysregulated expression of basic fibroblast growth factor [fibroblast growth factor 2 (FGF-2)] mediates autocrine growth of melanoma cells. The presence of a consensus Myb binding site in the human FGF-2 promoter prompted us to investigate whether this transcription factor could regulate FGF-2 expression in melanomas. We report that c-MYB mRNA is overexpressed in melanoma cell lines compared to normal melanocytes and that ectopic expression of murine c-Myb in SK-MEL-2 human melanoma cells resulted in increased expression of FGF-2 mRNA and FGF-2 protein. Furthermore, murine c-Myb transactivated a reporter plasmid containing the human FGF-2 promoter region in contransfected SK-MEL-2 human melanoma cells. Although a functional DNA-binding domain was required for transactivation, responsiveness to c-Myb was independent of the putative Myb binding site and mapped to two regions of the FGF-2 promoter that did not bind c-Myb in vitro. We suggest that c-MYB contributes to FGF-2-mediated autocrine growth of melanomas by indirectly regulating the FGF-2 promoter.
M R Miglarese; R Halaban; N W Gibson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research     Volume:  8     ISSN:  1044-9523     ISO Abbreviation:  Cell Growth Differ.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1997-12-30     Completed Date:  1997-12-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9100024     Medline TA:  Cell Growth Differ     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1199-210     Citation Subset:  IM    
Department of Cancer, Immunology and Infectious Diseases, Pfizer Central Research, Groton, Connecticut 06340, USA.
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MeSH Terms
Binding Sites
DNA-Binding Proteins / genetics,  metabolism*
Fibroblast Growth Factor 2 / biosynthesis*,  genetics
Melanoma / metabolism*
Promoter Regions, Genetic
Protein-Tyrosine Kinases / genetics,  metabolism*
Proto-Oncogene Proteins / genetics,  metabolism*
Proto-Oncogene Proteins c-myb
RNA, Messenger / metabolism
Trans-Activators / genetics,  metabolism*
Transcriptional Activation
Grant Support
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-myb; 0/RNA, Messenger; 0/Trans-Activators; 103107-01-3/Fibroblast Growth Factor 2; EC Kinases

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