Document Detail


Regulation of fetal lung disaturated phosphatidylcholine synthesis by de novo palmitate supply.
MedLine Citation:
PMID:  3334868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lung surfactant disaturated phosphatidylcholine (PC) is highly dependent on the supply of palmitate as a source of fatty acid. The purpose of this study was to investigate the importance of de novo fatty acid synthesis in the regulation of disaturated PC production during late prenatal lung development. Choline incorporation into disaturated PC and the rate of de novo fatty acid synthesis was determined by the relative incorporation of [14C]choline and 3H2O, respectively, in 20-day-old fetal rat lung explants and in 18-day-old explants which were cultured 2 days. Addition of exogenous palmitate (0.15 mM) increased (26%) choline incorporation into disaturated PC but did not inhibit de novo fatty acid synthesis, as classically seen in other lipogenic tissue. Even in the presence of exogenous palmitate, de novo synthesis accounted for 87% of the acyl groups for disaturated PC. Inhibition of fatty acid synthesis by agaric acid or levo-hydroxycitrate decreased the rate of choline incorporation into disaturated PC. When explants were subjected to both exogenous palmitate and 60% inhibition of de novo synthesis, disaturated PC synthesis was below control values and 75% of disaturated PC acyl moieties were still provided by de novo synthesis. These data show that surfactant disaturated PC synthesis is highly dependent on the supply of palmitate from de novo fatty acid synthesis.
Authors:
C E Patterson; K S Davis; R A Rhoades
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  958     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1988 Jan 
Date Detail:
Created Date:  1988-02-25     Completed Date:  1988-02-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  60-9     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis 46223.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Choline / metabolism
Fatty Acids / biosynthesis
Fetus
Kinetics
Lung / embryology,  metabolism*
Palmitic Acid
Palmitic Acids / metabolism*
Phosphatidylcholines / biosynthesis*
Rats
Rats, Inbred Strains
Grant Support
ID/Acronym/Agency:
HL 30355/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Palmitic Acids; 0/Phosphatidylcholines; 57-10-3/Palmitic Acid; 62-49-7/Choline

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