| Regulation of fetal lung disaturated phosphatidylcholine synthesis by de novo palmitate supply. | |
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MedLine Citation:
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PMID: 3334868 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lung surfactant disaturated phosphatidylcholine (PC) is highly dependent on the supply of palmitate as a source of fatty acid. The purpose of this study was to investigate the importance of de novo fatty acid synthesis in the regulation of disaturated PC production during late prenatal lung development. Choline incorporation into disaturated PC and the rate of de novo fatty acid synthesis was determined by the relative incorporation of [14C]choline and 3H2O, respectively, in 20-day-old fetal rat lung explants and in 18-day-old explants which were cultured 2 days. Addition of exogenous palmitate (0.15 mM) increased (26%) choline incorporation into disaturated PC but did not inhibit de novo fatty acid synthesis, as classically seen in other lipogenic tissue. Even in the presence of exogenous palmitate, de novo synthesis accounted for 87% of the acyl groups for disaturated PC. Inhibition of fatty acid synthesis by agaric acid or levo-hydroxycitrate decreased the rate of choline incorporation into disaturated PC. When explants were subjected to both exogenous palmitate and 60% inhibition of de novo synthesis, disaturated PC synthesis was below control values and 75% of disaturated PC acyl moieties were still provided by de novo synthesis. These data show that surfactant disaturated PC synthesis is highly dependent on the supply of palmitate from de novo fatty acid synthesis. |
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Authors:
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C E Patterson; K S Davis; R A Rhoades |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 958 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1988 Jan |
Date Detail:
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Created Date: 1988-02-25 Completed Date: 1988-02-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 60-9 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis 46223. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Choline / metabolism Fatty Acids / biosynthesis Fetus Kinetics Lung / embryology, metabolism* Palmitic Acid Palmitic Acids / metabolism* Phosphatidylcholines / biosynthesis* Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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HL 30355/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Palmitic Acids; 0/Phosphatidylcholines; 57-10-3/Palmitic Acid; 62-49-7/Choline |
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