| Regulation of ferritin levels in cultured lens epithelial cells. | |
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MedLine Citation:
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PMID: 9492756 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In most eukaryotic cells, synthesis of the iron storage protein, ferritin is regulated by iron levels and redox conditions. Proper iron storage is important to protect against damaging iron-catalysed free radical reactions. Although iron-catalysed reactions are believed to contribute to oxidative damage and cataractogenesis, little is known about iron storage in the lens. In this study, ferritin concentration was measured in cultured canine lens epithelial cells. Baseline ferritin concentration ranged from 76-163 ng (mg protein)-1; cells cultured in low-iron media had significantly lower ferritin levels than cells cultured in iron-supplemented media. Addition of a large excess of iron as hemin resulted in an eight-fold increase in ferritin concentration. The iron chelator, Desferal, significantly decreased ferritin concentration. The reducing agent dithiothreitol decreased the hemin-induced increase in ferritin levels, but not baseline levels. In contrast, ascorbic acid induced a large increase in ferritin content. Other studies have shown that induction of ferritin synthesis can protect against oxidative damage. Regulation of ferritin levels may represent a mechanism by which the lens epithelium is protected from oxidative damage. In vivo, epithelial cells are normally exposed to much lower iron concentrations than the cultured lens epithelial cells in this study. However, in pathological circumstances, the iron content and redox state of the aqueous humor is dramatically altered and may affect the steady state levels of ferritin within the lens. This remains to be determined. |
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Authors:
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M C McGahan; J Harned; A M Grimes; L N Fleisher |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental eye research Volume: 59 ISSN: 0014-4835 ISO Abbreviation: Exp. Eye Res. Publication Date: 1994 Nov |
Date Detail:
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Created Date: 1998-03-10 Completed Date: 1998-03-10 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370707 Medline TA: Exp Eye Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 551-5 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Physiology and Radiology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Chelating Agents / pharmacology Deferoxamine / pharmacology Dogs Enzyme-Linked Immunosorbent Assay Epithelial Cells / drug effects, metabolism* Ferritins / metabolism* Hemin / pharmacology Lens, Crystalline / cytology, drug effects, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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EY-04900/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chelating Agents; 16009-13-5/Hemin; 70-51-9/Deferoxamine; 9007-73-2/Ferritins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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