Document Detail


Regulation of expression of the cytochrome d terminal oxidase in Escherichia coli is transcriptional.
MedLine Citation:
PMID:  2828338     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cytochrome d complex is one of the two terminal oxidases in the aerobic respiratory system of Escherichia coli. This enzyme is not present in cells grown with high levels of dissolved oxygen in the culture medium but accumulates after mid-exponential growth, reaching high levels in stationary-phase cells. In this study, the transcriptional activity of the cyd operon, encoding the two subunits of the enzyme, was examined under a variety of growth conditions. This was accomplished by the use of a chromosomal operon fusion, cyd-lacZ, generated in vivo by a lambda plac-Mu hopper bacteriophage and also by the use of a cyd-lacZ protein fusion created in vitro on a plasmid, transferred onto a lambda transducing phage, and examined as a single-copy lysogen. Transcription of the gene fusions was monitored by determination of beta-galactosidase activity. The data clearly show that cyd is transcriptionally regulated and that induction is observed when the culture reaches a sufficient cell density so as to substantially reduce the steady-state levels of dissolved oxygen. The transcriptional activity is also regulated by other growth conditions, including the carbon source. The turn-on of cyd under semianaerobic conditions does not require the fnr gene product, cyclic AMP, or the cyclic AMP-binding protein.
Authors:
C D Georgiou; T J Dueweke; R B Gennis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  170     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1988 Feb 
Date Detail:
Created Date:  1988-03-10     Completed Date:  1988-03-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  961-6     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Illinois, Urbana 61801.
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MeSH Terms
Descriptor/Qualifier:
Aerobiosis
Anaerobiosis
Bacteriophage lambda
Chromosome Mapping
Cloning, Molecular
Cyclic AMP / metabolism
Cytochromes*
Electron Transport Chain Complex Proteins*
Escherichia coli / enzymology,  genetics*
Escherichia coli Proteins*
Gene Expression Regulation*
Operon*
Oxidoreductases / biosynthesis,  genetics*
Plasmids
Transcription, Genetic*
beta-Galactosidase / analysis
Grant Support
ID/Acronym/Agency:
HL16101/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Cytochromes; 0/Electron Transport Chain Complex Proteins; 0/Escherichia coli Proteins; 60-92-4/Cyclic AMP; EC 1.-/Oxidoreductases; EC 1.9.3.-/cytochrome bd terminal oxidase complex, E coli; EC 3.2.1.23/beta-Galactosidase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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