Document Detail

Regulation of excitatory transmission at hippocampal synapses by calbindin D28k.
MedLine Citation:
PMID:  7761464     Owner:  NLM     Status:  MEDLINE    
Distinct subpopulations of neurons in the brain contain one or more of the Ca(2+)-binding proteins calbindin D28k, calretinin, and parvalbumin. Although it has been shown that these high-affinity Ca(2+)-binding proteins can increase neuronal Ca2+ buffering capacity, it is not clear which aspects of neuronal physiology they normally regulate. To investigate this problem, we used a recently developed method for expressing calbindin D28k in the somatic and synaptic regions of cultured hippocampal pyramidal neurons. Ninety-six hours after infection with a replication-defective adenovirus containing the calbindin D28k gene, essentially all cultured hippocampal pyramidal neurons robustly expressed calbindin D28k. Our results demonstrate that while calbindin D28k does not alter evoked neurotransmitter release at excitatory pyramidal cell synapses, this protein has a profound effect on synaptic plasticity. In particular, we show that calbindin D28k expression suppresses posttetanic potentiation.
P S Chard; J Jordán; C J Marcuccilli; R J Miller; J M Leiden; R P Roos; G D Ghadge
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  92     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-06-29     Completed Date:  1995-06-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5144-8     Citation Subset:  IM    
Department of Pharmacological and Physiological Sciences, University of Chicago IL 60637, USA.
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MeSH Terms
Calcium-Binding Protein, Vitamin D-Dependent / biosynthesis,  metabolism*
Cell Line
Cells, Cultured
Electric Stimulation
Evoked Potentials* / drug effects
Gene Expression
Hippocampus / physiology*
N-Methylaspartate / pharmacology
Nerve Tissue Proteins / metabolism
Pyramidal Cells / physiology*
Recombinant Proteins / biosynthesis,  metabolism
Synapses / physiology*
Synaptic Transmission / drug effects,  physiology*
Time Factors
Grant Support
Reg. No./Substance:
0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Nerve Tissue Proteins; 0/Recombinant Proteins; 0/calbindin; 6384-92-5/N-Methylaspartate; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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